PHB3 通过 NO 介导的 AUXIN/INDOLE-3-ACETIC ACID 蛋白降解来调节侧根原基的形成。

PHB3 regulates lateral root primordia formation via NO-mediated degradation of AUXIN/INDOLE-3-ACETIC ACID proteins.

机构信息

State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, China.

College of Food Science and Engineering, Shandong Agricultural University, Tai'an, Shandong, China.

出版信息

J Exp Bot. 2022 Jun 24;73(12):4034-4045. doi: 10.1093/jxb/erac115.

DOI:10.1093/jxb/erac115

PMID:35303089

Abstract

We have previously shown that Arabidopsis thaliana Prohibitin 3 (PHB3) controls auxin-stimulated lateral root (LR) formation; however, the underlying molecular mechanism is unknown. Here, we demonstrate that PHB3 regulates lateral root (LR) development mainly through influencing lateral root primordia (LRP) initiation, via affecting nitric oxide (NO) accumulation. The reduced LRP in phb3 mutant was largely rescued by treatment with a NO donor. The decreased NO accumulation in phb3 caused a lower expression of GATA TRANSCRIPTION FACTOR 23 (GATA23) and LATERAL ORGAN BOUNDARIES DOMAIN 16 (LBD16) through inhibiting the degradation of INDOLE-3-ACETIC ACID INDUCIBLE 14/28 (IAA14/28). Overexpression of either GATA23 or LBD16 in phb3 mutant background recovered the reduced density of LRP. These results indicate that PHB3 regulates LRP initiation via NO-mediated auxin signalling, by modulating the degradation of IAA14/28.

摘要

我们之前已经证明，拟南芥 Prohibitin 3（PHB3）控制生长素刺激的侧根（LR）形成；然而，其潜在的分子机制尚不清楚。在这里，我们证明 PHB3 通过影响一氧化氮（NO）积累，主要通过影响侧根原基（LRP）起始来调节侧根（LR）的发育。用 NO 供体处理可大大挽救 phb3 突变体中减少的 LRP。phb3 引起的 NO 积累减少会通过抑制吲哚-3-乙酸诱导 14/28（IAA14/28）的降解，导致 GATA 转录因子 23（GATA23）和侧器官边界域 16（LBD16）的表达降低。在 phb3 突变体背景下过表达 GATA23 或 LBD16 均可恢复 LRP 密度的降低。这些结果表明，PHB3 通过调节 IAA14/28 的降解，通过 NO 介导的生长素信号转导调节 LRP 起始。