Nutritional Sciences, Division of Agriculture, Life and Veterinary Sciences, University of Arizona, Tucson, AZ 85721, USA.
Department of Chemistry and Biochemistry, College of Science, University of Arizona, Tucson, AZ 85721, USA.
J Insect Sci. 2022 Mar 1;22(2). doi: 10.1093/jisesa/ieac018.
Aedes aegypti [Linnaeus in Hasselquist; yellow fever mosquito] transmits several viruses that infect millions of people each year, including Zika, dengue, yellow fever, chikungunya, and West Nile. Pathogen transmission occurs during blood feeding. Only the females blood feed as they require a bloodmeal for oogenesis; in the bloodmeal, holo-transferrin and hemoglobin provide the females with a high iron load. We are interested in the effects of the bloodmeal on the expression of iron-associated proteins in oogenesis. Previous data showed that following digestion of a bloodmeal, ovarian iron concentrations doubles by 72 hr. We have used shotgun proteomics to identify proteins expressed in Ae. aegypti ovaries at two oogenesis developmental stages following blood feeding, and tandem mass tag-labeling proteomics to quantify proteins expressed at one stage following feeding of a controlled iron diet. Our findings provide the first report of mosquito ovarian protein expression in early and late oogenesis. We identify proteins differentially expressed in the two oogenesis development stages. We establish that metal-associated proteins play an important role in Ae. aegypti oogenesis and we identify new candidate proteins that might be involved in mosquito iron metabolism. Finally, this work identified a unique second ferritin light chain subunit, the first reported in any species. The shotgun proteomic data are available via ProteomeXchange with identifier PXD005893, while the tandem mass tag-labeled proteomic data are available with identifier PXD028242.
埃及伊蚊[林奈在哈塞尔奎斯特；黄热病蚊]传播几种病毒，每年感染数百万人，包括寨卡病毒、登革热、黄热病、基孔肯雅热和西尼罗河病毒。病原体传播发生在吸血时。只有雌性吸血，因为它们需要血液来进行卵子发生；在血液中，全转铁蛋白和血红蛋白为雌性提供高铁负荷。我们对血液对卵子发生中铁相关蛋白表达的影响感兴趣。先前的数据表明，在消化血液后，卵巢铁浓度在 72 小时内增加一倍。我们使用鸟枪法蛋白质组学来鉴定在吸血后两个卵子发生发育阶段的埃及伊蚊卵巢中表达的蛋白质，并使用串联质量标签标记蛋白质组学来定量在喂食受控铁饮食后的一个阶段中表达的蛋白质。我们的研究结果首次报道了蚊子卵巢在早期和晚期卵子发生中的蛋白质表达。我们确定了在两个卵子发生发育阶段表达差异的蛋白质。我们确定了金属相关蛋白在埃及伊蚊卵子发生中起着重要作用，并鉴定了可能参与蚊子铁代谢的新候选蛋白。最后，这项工作鉴定了一个独特的第二个铁蛋白轻链亚基，这是在任何物种中首次报道的。鸟枪法蛋白质组学数据可通过 ProteomeXchange 获得，标识符为 PXD005893，而串联质量标签标记蛋白质组学数据可通过标识符 PXD028242 获得。
