Chen Yue, Wang Jinpeng, Zhao Yang-Guo, Maqbool Farhana, Gao Mengchun, Guo Liang, Ji Junyuan, Zhao Xuning, Zhang Mo
Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering (MEGE), College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, China.
Shandong Provincial Key Laboratory of Marine Environment and Geological Engineering (MEGE), College of Environmental Science and Engineering, Ocean University of China, Qingdao, 266100, China; Key Lab of Marine Environmental Science and Ecology (Ocean University of China), Ministry of Education, Qingdao, 266100, China.
Chemosphere. 2022 Jul;298:134327. doi: 10.1016/j.chemosphere.2022.134327. Epub 2022 Mar 15.
Antibiotics are widely dosed in mariculture sector, resulting in substantial antibiotics residues. Hence, mariculture wastewater is urgent to be treated before discharging. In this study, the anoxic/oxic moving bed biofilm reactor (A/O-MBBR) was used to treat the wastewater containing sulfamethoxazole (SMX) from mariculture, SMX removal mechanism and the variation of antibiotic-resistant genes (ARGs) were investigated. The results showed that 22%-33% of SMX was removed by the bioreactor, where a small amount of SMX was adsorbed and stored by the extracellular polymers and most of SMX (>80%) was biodegraded in the anoxic tank. Occurrence of nitrate in anoxic condition was conducive to SMX degradation. Pseudomonas, Desulfuromusa, and Methanolobus species, as well as microbial catalase contributed to the SMX biotransformation. Quantitative PCR analysis of ARGs (sul1, sul2 and int1) and mRNA (sul1, sul2) showed that SMX enriched SMX-related ARGs and enhanced the expression of corresponding genes. Most of ARGs finally were discharged with effluent. Hence, the effluent from biologically based processes treating mariculture wastewater still contained antibiotics residue and resistance genes, which should be further controlled by suitable techniques.
抗生素在海水养殖行业中大量使用，导致大量抗生素残留。因此，海水养殖废水在排放前急需处理。本研究采用缺氧/好氧移动床生物膜反应器（A/O-MBBR）处理海水养殖中含磺胺甲恶唑（SMX）的废水，研究了SMX的去除机制及抗生素抗性基因（ARGs）的变化。结果表明，生物反应器去除了22%-33%的SMX，其中少量SMX被细胞外聚合物吸附和储存，大部分SMX（>80%）在缺氧池中被生物降解。缺氧条件下硝酸盐的存在有利于SMX的降解。假单胞菌属、脱硫穆尔氏菌属和嗜甲基菌属以及微生物过氧化氢酶有助于SMX的生物转化。对ARGs（sul1、sul2和int1）和mRNA（sul1、sul2）的定量PCR分析表明，SMX富集了与SMX相关的ARGs并增强了相应基因的表达。大多数ARGs最终随废水排放。因此，基于生物处理的海水养殖废水处理后的出水仍含有抗生素残留和抗性基因，应采用合适的技术进一步加以控制。
