Kelsoe G, Stout J T
Cell Immunol. 1986 Apr 1;98(2):506-16. doi: 10.1016/0008-8749(86)90309-6.
Paratopic frequencies of C57BL/6 (Igh-Vb) and BALB/c (Igh-Va) mice were compared by determining the frequency of lipopolysaccharide-reactive, splenic B lymphocytes secreting antibody specific for (4-hydroxy-5-iodo-3-nitrophenyl) acetyl (NIP), trinitrophenyl (TNP), phosphorylcholine (PC), NIP/TNP, NIP/PC, and sheep erythrocytes. Despite the known genotypic and phenotypic differences between the two Igh-V loci, no significant differences in paratope frequencies were demonstrated. Similar determinations in C.B-20 mice, Ighb congenics of the BALB/c strain, and in C57BL/10 nude mice indicated that the mitogen-generated paratope frequencies directly reflected the capacity of immunoglobulin variable region elements rather than complex interactive or regulatory controls to generate diversity. We conclude that at least for the paratopic repertoire, the role of the somatic processes for the generation of antibody diversity exceeds the influence of germ-line differences between the Ighb and Igha haplotypes.
通过测定分泌针对（4-羟基-5-碘-3-硝基苯基）乙酰基（NIP）、三硝基苯基（TNP）、磷酸胆碱（PC）、NIP/TNP、NIP/PC和绵羊红细胞的抗体的脂多糖反应性脾B淋巴细胞的频率，比较了C57BL/6（Igh-Vb）和BALB/c（Igh-Va）小鼠的互补决定区频率。尽管已知两个Igh-V基因座之间存在基因型和表型差异，但未显示互补决定区频率有显著差异。在C.B-20小鼠（BALB/c品系的Ighb同源基因）和C57BL/10裸鼠中进行的类似测定表明，丝裂原产生的互补决定区频率直接反映了免疫球蛋白可变区元件的能力，而不是产生多样性的复杂相互作用或调节控制。我们得出结论，至少对于互补决定区库而言，体细胞过程在产生抗体多样性方面的作用超过了Ighb和Igha单倍型之间种系差异的影响。
