Chow S N, Hsieh C Y, Ouyang P C, Leung W Y, Ho-Yuen B, Lee C Y
Diagn Immunol. 1986;4(4):194-200.
Monoclonal antibodies generated against human chorionic gonadotropin (hCG) were utilized in radio- and enzyme-immunoassays of this reproductive hormone in biological fluids. One of the monoclonal antibodies, beta-4D6, was shown to have extremely high affinity (Ka = 8 X 10(10) M-1)) and high specificity (less than or equal to 0.6% LH cross-reactivity) to beta-subunit of hCG and whole hCG. It was used in competitive radioimmunoassays (RIA) for the determination of low levels of serum hCG. An excellent correlation was obtained concerning the assay results between the monoclonal antibody-based system and others using conventional polyclonal anti-sera. In combination with another discrete hCG monoclonal antibody, solid-phase sandwich radiometric and enzyme immunoassays were established. These immunoassays could easily be performed, and they offer sensitivity and efficacy of hCG determination comparable to those of conventional ones.
针对人绒毛膜促性腺激素（hCG）产生的单克隆抗体被用于生物体液中这种生殖激素的放射免疫分析和酶免疫分析。其中一种单克隆抗体β-4D6，对hCG的β亚基和完整hCG显示出极高的亲和力（Ka = 8×10¹⁰ M⁻¹）和高特异性（LH交叉反应性小于或等于0.6%）。它被用于竞争性放射免疫分析（RIA）以测定血清中低水平的hCG。基于单克隆抗体的系统与使用传统多克隆抗血清的其他系统之间的检测结果具有良好的相关性。与另一种不同的hCG单克隆抗体相结合，建立了固相夹心放射测量法和酶免疫分析法。这些免疫分析方法操作简便，在hCG检测方面提供了与传统方法相当的灵敏度和效能。
