Hu Xiaoxian, Liu Yaofa, Zhu Dandan, Jin Yongguo, Jin Haobo, Sheng Long
National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
National Research and Development Center for Egg Processing, College of Food Science and Technology, Huazhong Agricultural University, Wuhan, Hubei 430070, PR China.
Food Chem. 2022 Aug 15;385:132708. doi: 10.1016/j.foodchem.2022.132708. Epub 2022 Mar 15.
Carboxymethyl cellulose (CMC) films containing lysozyme (Lys) were prepared in this study and changes in properties of the films were investigated. Enhancement in mechanical properties was observed with increased Lys, maximum (0.05 g/100 mL) reached to 39.07 MPa (TS) and 25.04 % (EAB). Meanwhile, water resistance ability improved, the minimum (0.05 g/100 mL) reached to 0.42 g·mm·(m·h·KPa), 84.62 % of pure CMC film. Thermogravimetric test showed better thermal stability of films. Scanning electron microscope illustrated that few cracks on surface of films. Fourier Transform infrared spectroscopy supported that more intermolecular hydrogen between Lys and CMC was formed with increased Lys, yet keeping increasing formed less intermolecular hydrogen. X-ray Diffraction observed the aggregated Lys by crystal structure. Antibacterial test showed an inhibitory effect on two common food-borne pathogens. Weight loss experiment indicated that films reduced the dry consumption of meat. Overall, the modification of CMC film by adding Lys was effective.
本研究制备了含有溶菌酶（Lys）的羧甲基纤维素（CMC）薄膜，并对薄膜的性能变化进行了研究。随着Lys含量增加，薄膜的机械性能得到增强，当Lys含量达到最大值（0.05 g/100 mL）时，拉伸强度（TS）达到39.07 MPa，断裂伸长率（EAB）达到25.04%。同时，耐水性提高，当Lys含量为最小值（0.05 g/100 mL）时，透湿系数达到0.42 g·mm·(m·h·KPa)，为纯CMC薄膜的84.62%。热重测试表明薄膜具有更好的热稳定性。扫描电子显微镜显示薄膜表面有少量裂纹。傅里叶变换红外光谱表明，随着Lys含量增加，Lys与CMC之间形成了更多的分子间氢键，但继续增加Lys含量时，分子间氢键的形成量减少。X射线衍射观察到Lys以晶体结构聚集。抗菌测试表明该薄膜对两种常见的食源性病原体有抑制作用。失重实验表明薄膜减少了肉类的干耗。总体而言，通过添加Lys对CMC薄膜进行改性是有效的。
