开发并验证了一种使用核壳柱和邻苯二醛柱后衍生化的直接 HPLC 方法，用于测定唾液中二硫键谷胱甘肽。
Development and validation of a direct HPLC method for the determination of salivary glutathione disulphide using a core shell column and post column derivatization with o-phthalaldehyde.
机构信息
Laboratory of Analytical Chemistry, School of Chemistry, Faculty of Sciences, Aristotle University of Thessaloniki, GR-54124, Greece.
Laboratory of Pharmaceutical Analysis, Department of Pharmaceutical Technology, School of Pharmacy, Aristotle University of Thessaloniki, GR-54124, Greece.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2022 May 1;1197:123216. doi: 10.1016/j.jchromb.2022.123216. Epub 2022 Mar 16.
Glutathione disulfide (GSSG) has been monitored in human saliva samples by an optimized and validated method that is based on liquid chromatography coupled to on-line post column derivatization. The analyte was separated from the sample matrix using a 100% aqueous mobile phase through a core-shell reversed phase column. Following optimization of the reaction using Box- Behnken experimental design and validation, GSSG was quantified accurately and selectively in the range of 100-2000 nmol L with a LOD of 20 nmol L. GSSG was quantified in 15 out of 20 human saliva samples (75%) with a mean value of 860 nmol L (150-4600 nmol L). Blocking of reduced Glutathione with N-ethylmaleimide ensured stability of the samples for at least 72 h at all temperatures examined.
谷胱甘肽二硫化物（GSSG）已通过一种优化和验证的方法在人唾液样本中进行监测，该方法基于液相色谱与在线柱后衍生化相结合。通过核壳反相柱，使用 100%水流动相从样品基质中分离分析物。在使用 Box-Behnken 实验设计进行反应优化和验证后，GSSG 在 100-2000nmol L 的范围内被准确且选择性地定量，检出限为 20nmol L。在 20 个人唾液样本中的 15 个（75%）中定量了 GSSG，平均值为 860nmol L（150-4600nmol L）。用 N-乙基马来酰亚胺封闭还原型谷胱甘肽可确保样品在所有检查温度下至少 72 小时的稳定性。
Zotero 插件