Graduate Program in Human Aging, University of Passo Fundo, Passo Fundo, Rio Grande do Sul, Brazil.
Clinical Hospital of Passo Fundo, Passo Fundo, Rio Grande do Sul, Brazil.
Knee. 2022 Mar;35:114-123. doi: 10.1016/j.knee.2022.03.003. Epub 2022 Mar 17.
Osteoarthritis (OA) is a joint disease of multifactorial etiology, affecting mainly the knees. We aimed to evaluate the effects of two different doses of gaseous ozone intra-articularly on the knee cartilage morphology of rats with osteoarthritis (OA).
The articular lesion was induced by sodium monoiodoacetate (MIA). 40 Wistar rats were divided into 4 groups: G1 control (without lesion and without treatment), G2 articular lesion (AL) (only lesion MIA-induced), G3 AL + treatment with 5 μg/mL of ozone intra-articular, and G4 AL + treatment with 10 μg/mL of ozone intra-articular. The experiment was carried out for 60 days.
Both doses of ozone intra-articular demonstrated less reduction in joint space (G3 and G4) compared to the G2, formation of osteophytes, but without subchondral sclerosis. Ozone decreased the volumetric density of the articular lesion (VV(AL)) of tibial. The treatments recovered VV(AL) of the femur similar to G1. Ozone lower dose (G3) showed lower tibia and femur macroscopic scores.
Intra-articular gaseous ozone can delay the degeneration of articular cartilage and can represents an integrative therapy in the OA treatment of knee after 60 days of treatment. For the first time the role of ozone in articular cartilage degeneration was evaluated helping to understand this therapy.
骨关节炎（OA）是一种多因素病因的关节疾病，主要影响膝关节。我们旨在评估两种不同剂量的气态臭氧关节内注射对骨关节炎（OA）大鼠膝关节软骨形态的影响。
关节损伤由单碘乙酸钠（MIA）诱导。将 40 只 Wistar 大鼠分为 4 组：G1 对照组（无损伤且无治疗）、G2 关节损伤（AL）组（仅诱导 MIA 损伤）、G3 AL+5μg/mL 臭氧关节内治疗组和 G4 AL+10μg/mL 臭氧关节内治疗组。实验进行了 60 天。
与 G2 组相比，两种剂量的臭氧关节内注射均能减少关节间隙的减少（G3 和 G4）、骨赘形成，但无软骨下硬化。臭氧降低了胫骨的关节病变体积密度（VV（AL））。治疗后，臭氧恢复了股骨的 VV（AL），与 G1 相似。臭氧低剂量（G3）组的胫骨和股骨宏观评分较低。
关节内气态臭氧可以延缓关节软骨的退化，在膝关节 OA 治疗 60 天后可以作为一种综合治疗方法。这是首次评估臭氧在关节软骨退化中的作用，有助于了解这种治疗方法。
