Lauberte Liga, Ponomarenko Jevgenija, Arshanitsa Alexander
Latvian State Institute of Wood Chemistry, Dzerbenes Str., 27, LV 1006 Riga, Latvia.
Latvian State Institute of Wood Chemistry, Dzerbenes Str., 27, LV 1006 Riga, Latvia.
J Pharm Biomed Anal. 2022 May 30;214:114727. doi: 10.1016/j.jpba.2022.114727. Epub 2022 Mar 15.
An experimentally proven novel analytical approach for chromatographic analysis of diarylheptanoids in grey alder (Alnus incana) and black alder (Alnus glutinosa) bark matrices was established. A method for qualitative and quantitative determination of oregonin (dominant diarylheptanoid) and semiquantitative analysis of related diarylheptanoids from alder bark using a photodiode array (PDA) detector coupled to a high-resolution mass spectrometer (QTOF-MS) was developed. A comparison of different liquid chromatography detectors (UV, MS and ELS) showed that only a combination of them is applicable for comprehensive analysis of multicomponent extracts. A total of sixteen different diarylheptanoids were simultaneously identified and semi-quantified in alder bark extracts. This is the first report of the method for individual and total diarylheptanoid determination in alder bark extracts, discussed in detail. The liquid chromatography complex is suggested as a tool for the reliable identification and quality control of the diarylheptanoids containing extracts isolated from the Alnus species and their dominant component - oregonin. The semiquantitative methodology established, and the dominant compound quantification provided means for assessing comparative sample complexities.
建立了一种经实验验证的新型分析方法，用于对灰桤木（Alnus incana）和黑桤木（Alnus glutinosa）树皮基质中的二芳基庚烷类化合物进行色谱分析。开发了一种使用与高分辨率质谱仪（QTOF-MS）联用的光电二极管阵列（PDA）检测器对桤木树皮中的俄勒冈宁（主要的二芳基庚烷类化合物）进行定性和定量测定以及对相关二芳基庚烷类化合物进行半定量分析的方法。对不同液相色谱检测器（紫外、质谱和蒸发光散射）的比较表明，只有将它们组合起来才适用于多组分提取物的综合分析。在桤木树皮提取物中同时鉴定并半定量了总共16种不同的二芳基庚烷类化合物。本文详细讨论了桤木树皮提取物中二芳基庚烷类化合物的单独测定和总量测定方法的首次报道。建议将液相色谱组合作为一种工具，用于可靠鉴定和质量控制从桤木属植物中分离得到的含二芳基庚烷类化合物的提取物及其主要成分——俄勒冈宁。所建立的半定量方法以及主要化合物的定量为评估比较样品的复杂性提供了手段。
