Screening method for chromatographic analysis of diarylheptanoids in alder bark extracts.

An experimentally proven novel analytical approach for chromatographic analysis of diarylheptanoids in grey alder (Alnus incana) and black alder (Alnus glutinosa) bark matrices was established. A method for qualitative and quantitative determination of oregonin (dominant diarylheptanoid) and semiquantitative analysis of related diarylheptanoids from alder bark using a photodiode array (PDA) detector coupled to a high-resolution mass spectrometer (QTOF-MS) was developed. A comparison of different liquid chromatography detectors (UV, MS and ELS) showed that only a combination of them is applicable for comprehensive analysis of multicomponent extracts. A total of sixteen different diarylheptanoids were simultaneously identified and semi-quantified in alder bark extracts. This is the first report of the method for individual and total diarylheptanoid determination in alder bark extracts, discussed in detail. The liquid chromatography complex is suggested as a tool for the reliable identification and quality control of the diarylheptanoids containing extracts isolated from the Alnus species and their dominant component - oregonin. The semiquantitative methodology established, and the dominant compound quantification provided means for assessing comparative sample complexities.