在白斑狗鱼（Esox lucius）中，抗缪勒氏管激素2型受体（amhrII）基因的失活导致雄性向雌性的性逆转。

Inactivation of the Anti-Müllerian Hormone Receptor Type 2 (amhrII) Gene in Northern Pike (Esox lucius) Results in Male-To-Female Sex Reversal.

作者信息

Pan Qiaowei, Herpin Amaury, Guiguen Yann

机构信息

INRAE, LPGP, Rennes, France.

Department of Ecology and Evolution, University of Lausanne, Lausanne, Switzerland.

出版信息

Sex Dev. 2022;16(4):289-294. doi: 10.1159/000521003. Epub 2022 Mar 18.

DOI:10.1159/000521003

Abstract

BACKGROUND

The anti-müllerian hormone (Amh) pathway is crucial for sexual development in teleosts. A male-specific duplicate of anti-müllerian hormone (amhby) was previously identified as the northern pike (Esox lucius) master sex determination gene. However, the role of its putative cognate receptor, i.e., the anti-müllerian hormone receptor type 2 (amhrII) was unclear in this species.

OBJECTIVE

Here, we investigated the role of amhrII during sexual development of northern pike.

METHOD

We generated stable mutants with deletions in exon 9 of amhrII, inactivating the AmhrII protein using a CRISPR-Cas9-mediated gene knockout strategy.

RESULT

The inactivation of amhrII in northern pike results in a high level of male-to-female sex reversal.

CONCLUSION

This result demonstrates that amhrII is necessary for male sexual development in northern pike and supports the idea that AmhrII is a conserved regulator of the teleosts sex differentiation network.

摘要

背景

抗缪勒氏管激素（Amh）信号通路对硬骨鱼的性别发育至关重要。抗缪勒氏管激素的雄性特异性复制基因（amhby）先前被确定为白斑狗鱼（Esox lucius）的主要性别决定基因。然而，其假定的同源受体，即抗缪勒氏管激素2型受体（amhrII）在该物种中的作用尚不清楚。

目的

在此，我们研究了amhrII在白斑狗鱼性别发育过程中的作用。

方法

我们利用CRISPR-Cas9介导的基因敲除策略，构建了amhrII外显子9缺失的稳定突变体，使AmhrII蛋白失活。

结果

白斑狗鱼中amhrII的失活导致了高水平的雄性向雌性的性逆转。

结论

这一结果表明，amhrII是白斑狗鱼雄性性别发育所必需的，并支持AmhrII是硬骨鱼性别分化网络保守调节因子的观点。