欧前胡素联合放疗或热疗抑制 MCF-7 细胞的增殖。

Imperatorin Attenuates the Proliferation of MCF-7 Cells in Combination with Radiotherapy or Hyperthermia.

机构信息

Department of Radiology, Faculty of Paramedical, Tehran University of Medical Sciences, Tehran, Iran.

Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.

出版信息

Curr Radiopharm. 2022;15(3):236-241. doi: 10.2174/1874471015666220318122202.

DOI:10.2174/1874471015666220318122202

PMID:35306999

Abstract

BACKGROUND

Breast cancer is one of the most common types of malignancies in the world. Cancer resistance is an unavoidable consequence of therapy with radiation or other modalities. Ongoing research aims to improve cancer response to therapy.

AIM

The aim of this study was to evaluate the possible sensitization effect of imperatorin (IMP) in combination with external radiotherapy (ERT) or HT.

METHODS

After treatment of MCF-7 breast cancer cells with IMP, cells were exposed to 4 Gy X-rays or HT (42 °C for 1 hour). The viability of MCF-7 cells was measured using an MTT assay. Furthermore, the expression of pro-apoptotic genes, including Bax, Bcl-2, caspase-3, caspase-8, and caspase- 9, was investigated using real-time PCR. The sensitizing effect of IMP in combination with ERT or HT was calculated and compared to ERT or HT alone.

RESULTS

Results showed an increase in the expression of pro-apoptotic genes and downregulation of anti-apoptotic Bcl-2 following ERT and HT. Furthermore, cell viability was reduced following these treatments. IMP was able to augment these effects of ERT and HT.

CONCLUSION

IMP could increase the efficiency of HT and ERT. This effect of IMP may suggest it as an adjuvant for increasing the therapeutic efficiency of ERT.

摘要

背景

乳腺癌是世界上最常见的恶性肿瘤之一。癌症耐药性是放射治疗或其他治疗方式不可避免的结果。目前的研究旨在提高癌症对治疗的反应。

目的

本研究旨在评估欧前胡素（IMP）联合外部放射治疗（ERT）或 HT 是否具有潜在的增敏作用。

方法

用 IMP 处理 MCF-7 乳腺癌细胞后，将细胞暴露于 4 Gy X 射线或 HT（42°C 1 小时）。使用 MTT 法测量 MCF-7 细胞的活力。此外，使用实时 PCR 研究包括 Bax、Bcl-2、caspase-3、caspase-8 和 caspase-9 在内的促凋亡基因的表达。计算并比较 IMP 联合 ERT 或 HT 与单独 ERT 或 HT 的增敏作用。