Seyedpour Nasrin, Motevaseli Elahe, Taeb Shahram, Nowrouzi Azin, Mirzaei Fatemeh, Bahri Mina, Dehghan-Manshadi Hamid Reza, Zhaleh Mohsen, Rashidi Khodabakhsh, Azmoonfar Rasool, Yahyapour Rasoul, Najafi Masoud
Department of Medical Physics and Biomedical Engineering, Tehran University of Medical Sciences, Tehran, Iran.
Nanomedicine Research Association (NRA), Universal Scientific Education and Research Network (USERN), Tehran, Iran.
Curr Radiopharm. 2024;17(1):99-110. doi: 10.2174/0118744710244357231018070313.
This study investigated the protective effects of three antioxidants on radiationinduced lung injury.
Oxidative stress is one of the key outcomes of radiotherapy in normal tissues. It can induce severe injuries in lung tissue, which may lead to pneumonitis and fibrosis. Recently, interest in natural chemicals as possible radioprotectors has increased due to their reduced toxicity, cheaper price, and other advantages.
The present study was undertaken to evaluate the radioprotective effect of Alpha-lipoic Acid (LA), Resveratrol (RVT), and Apigenin (APG) against histopathological changes and oxidative damage and survival induced by ionizing radiation (IR) in the lung tissues of rats.
First, the lung tissue of 50 mature male Wistar rats underwent an 18 Gy gamma irradiation. Next, the rats were sacrificed and transverse sections were obtained from the lung tissues and stained with hematoxylin and eosin (H and E) and Mason trichrome (MTC) for histopathological evaluation. Then, the activity of Glutathione peroxidase (GPx), Superoxide Dismutase (SOD), and Malondialdehyde (MDA) was measured by an ELISA reader at 340, 405, and 550 nm.
Based on the results of this study, IR led to a remarkable increase in morphological changes in the lung. However, APG, RVT, and LA could ameliorate the deleterious effects of IR in lung tissue. IR causes an increase in GPX level, and APG+IR administration causes a decrease in the level of GPX compared to the control group. Also, the results of this study showed that RVT has significant effects in reducing MDA levels in the short term. In addition, compared to the control group, IR and RVT+IR decrease the activity of SOD in the long term in the lung tissues of rats. Also, the analysis of results showed that weight changes in IR, LA+IR, APG+IR, and control groups were statistically significant.
APG and RVT could prevent tissue damage induced by radiation effects in rat lung tissues. Hence, APG, LA, and RVT could provide a novel preventive action with their potential antioxidant anti-inflammatory properties, as well as their great safety characteristic.
本研究调查了三种抗氧化剂对辐射诱导的肺损伤的保护作用。
氧化应激是正常组织放疗的关键后果之一。它可导致肺组织严重损伤，进而可能引发肺炎和纤维化。近年来，由于天然化学物质毒性较低、价格便宜及其他优势，人们对其作为潜在辐射防护剂的兴趣日益增加。
本研究旨在评估α-硫辛酸（LA）、白藜芦醇（RVT）和芹菜素（APG）对大鼠肺组织电离辐射（IR）诱导的组织病理学变化、氧化损伤及生存的辐射防护作用。
首先，对50只成年雄性Wistar大鼠的肺组织进行18 Gy的γ射线照射。接下来，处死大鼠，从肺组织获取横切面，并用苏木精和伊红（H&E）及马森三色染色法（MTC）进行组织病理学评估。然后，用酶标仪在340、405和550 nm处测量谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）和丙二醛（MDA）的活性。
基于本研究结果，IR导致肺组织形态学变化显著增加。然而，APG、RVT和LA可改善IR对肺组织的有害影响。IR导致GPX水平升高，与对照组相比，APG + IR给药导致GPX水平降低。此外，本研究结果表明，RVT在短期内对降低MDA水平有显著作用。另外，与对照组相比，IR和RVT + IR长期降低大鼠肺组织中SOD的活性。而且，结果分析表明，IR、LA + IR、APG + IR组和对照组的体重变化具有统计学意义。
APG和RVT可预防大鼠肺组织辐射效应诱导的组织损伤。因此，APG、LA和RVT凭借其潜在的抗氧化抗炎特性以及良好的安全性，可提供一种新的预防作用。
