Engelking L E, Ambrose D J, Oba M
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.
Department of Agricultural, Food and Nutritional Science, University of Alberta, Edmonton, Alberta, Canada T6G 2P5.
J Dairy Sci. 2022 May;105(5):4144-4155. doi: 10.3168/jds.2021-21553. Epub 2022 Mar 17.
Dairy cattle experience inflammation during the calving transition period, and butyrate and nonsteroidal anti-inflammatory drugs (NSAID) are expected to reduce the inflammation. Our objective was to evaluate the effects of dietary butyrate supplementation and oral NSAID administration on feed intake, serum inflammatory markers, plasma metabolites, and milk production of dairy cows during the calving transition period. Eighty-three Holstein cows were used in the experiment with a 2 × 2 factorial arrangement of treatments. The cows were blocked by parity and calving date, and randomly assigned to a dietary butyrate or control supplement, and NSAID or a placebo oral administration. Experimental diets were iso-energetic containing calcium butyrate at 1.42% of diet dry matter (DM) or the control supplement (1.04% commercial fat supplement and 0.38% calcium carbonate of diet DM). The close-up diets contained 13.3% starch and 42.4% neutral detergent fiber on a DM basis, and were fed from 28 d before expected calving date until calving. The postpartum diets contained 22.1% starch and 34.1% neutral detergent fiber on a DM basis and were fed from calving to 24 d after calving. Oral NSAID (1 mg of meloxicam/kg of body weight) or placebo (food dye) was administered 12 to 24 h after calving. Dietary butyrate supplementation and oral NSAID administration did not affect milk yield or postpartum serum concentrations of amyloid A and haptoglobin. However, butyrate-fed cows increased plasma fatty acid concentration on d -4 relative to calving (501 vs. 340 μEq/L) and tended to increase serum haptoglobin concentration (0.23 vs. 0.10 mg/mL). There was a supplement by drug interaction effect on plasma glucose concentration on d 4; in cows administered the placebo drug, butyrate supplementation decreased plasma glucose concentration compared with control-fed cows (62.8 vs. 70.1 mg/dL). Butyrate-fed cows tended to have lower milk crude protein yield compared with cows fed the control diet (1.21 vs. 1.27 kg/d). Dietary butyrate supplementation and oral NSAID administration did not have overall positive effects on production performance of dairy cows during the calving transition period.
奶牛在产犊过渡期会经历炎症，丁酸盐和非甾体抗炎药（NSAID）有望减轻炎症。我们的目标是评估在产犊过渡期，日粮中添加丁酸盐和口服NSAID对奶牛采食量、血清炎症标志物、血浆代谢物和产奶量的影响。本实验使用了83头荷斯坦奶牛，采用2×2析因设计处理。奶牛按胎次和产犊日期进行分组，随机分配到日粮丁酸盐或对照补充剂组，以及NSAID或安慰剂口服给药组。实验日粮能量相等，含1.42%日粮干物质（DM）的丁酸钠或对照补充剂（1.04%商业脂肪补充剂和0.38%日粮DM的碳酸钙）。围产前期日粮以DM计含13.3%淀粉和42.4%中性洗涤纤维，从预计产犊日期前28天喂至产犊。产后日粮以DM计含22.1%淀粉和34.1%中性洗涤纤维，从产犊喂至产后24天。产犊后12至24小时口服NSAID（1毫克美洛昔康/千克体重）或安慰剂（食用色素）。日粮中添加丁酸盐和口服NSAID对产奶量或产后血清淀粉样蛋白A和触珠蛋白浓度没有影响。然而，与产犊相比，饲喂丁酸盐的奶牛在d -4时血浆脂肪酸浓度升高（501对340μEq/L），血清触珠蛋白浓度有升高趋势（0.23对0.10毫克/毫升）。在第4天，血浆葡萄糖浓度存在补充剂与药物的交互作用；在给予安慰剂药物的奶牛中，与饲喂对照日粮的奶牛相比，添加丁酸盐降低了血浆葡萄糖浓度（62.8对70.1毫克/分升）。与饲喂对照日粮的奶牛相比，饲喂丁酸盐的奶牛乳粗蛋白产量有降低趋势（1.21对1.27千克/天）。在产犊过渡期，日粮中添加丁酸盐和口服NSAID对奶牛生产性能没有总体积极影响。
