Orskov C, Holst J J, Knuhtsen S, Baldissera F G, Poulsen S S, Nielsen O V
Endocrinology. 1986 Oct;119(4):1467-75. doi: 10.1210/endo-119-4-1467.
We developed specific antibodies and RIAs for glucagon-like peptides 1 and 2 (GLP-1 and GLP-2), two predicted products of the glucagon gene, and studied the occurrence, nature, and secretion of immunoreactive GLP-1 and GLP-2 in pig pancreas and small intestine. Immunoreactive GLP-1 and GLP-2 were identified in glucagon-producing cells of the pancreatic islets, and in glicentin-producing cells of the small intestine. Immunoreactive GLP-1 and 2 in intestinal extracts corresponded in molecular size to peptides synthesized according to the predicted structure. By reverse phase HPLC, intestinal and synthetic GLP-1 behaved similarly, whereas synthetic and intestinal GLP-2 differed. Pancreatic extracts contained a large peptide with both GLP-1 and GLP-2 immunoreactivity. Secretion was studied using isolated perfused pig pancreas during arginine stimulation, and isolated perfused pig ileum during either luminal glucose stimulation or vascular administration of the neuropeptide, gastrin-releasing peptide (GRP). Immunoreactive GLP-1 and GLP-2 were secreted in parallel with pancreatic glucagon and intestinal glicentin. The molecular forms of secreted immunoreactive GLP-1 and 2 corresponded to those identified in the tissue extracts.
我们针对胰高血糖素基因的两个预测产物胰高血糖素样肽1和2（GLP-1和GLP-2）开发了特异性抗体和放射免疫分析法，并研究了猪胰腺和小肠中免疫反应性GLP-1和GLP-2的产生、性质及分泌情况。在胰岛的胰高血糖素产生细胞以及小肠的肠高血糖素产生细胞中鉴定出了免疫反应性GLP-1和GLP-2。小肠提取物中的免疫反应性GLP-1和2在分子大小上与根据预测结构合成的肽相对应。通过反相高效液相色谱法，小肠和合成的GLP-1表现相似，而合成的和小肠的GLP-2有所不同。胰腺提取物含有一种同时具有GLP-1和GLP-2免疫反应性的大肽。在精氨酸刺激期间使用离体灌注猪胰腺，以及在腔内葡萄糖刺激或神经肽胃泌素释放肽（GRP）血管给药期间使用离体灌注猪回肠来研究分泌情况。免疫反应性GLP-1和GLP-2与胰腺胰高血糖素和小肠肠高血糖素平行分泌。分泌的免疫反应性GLP-1和2的分子形式与在组织提取物中鉴定出的形式相对应。
