Hennemann G, Krenning E P, Polhuys M, Mol J A, Bernard B F, Visser T J, Docter R
Endocrinology. 1986 Oct;119(4):1870-2. doi: 10.1210/endo-119-4-1870.
We investigated if carrier-mediated transport into rat hepatocytes is rate-limiting in total cellular uptake and metabolism of thyroid hormone. Rat hepatocytes in primary monolayer culture were incubated under equilibrium conditions with tracer T4, T3 or rT3 in the absence or presence of inhibitors of thyroid hormone uptake, i.e., ouabain and ER-22, a monoclonal antibody against the rat hepatocyte plasma membrane. The results for all three iodothyronines show that inhibition of clearance from the medium during incubation is paralleled by a similar decrease in iodide production. This indicates that the decrease in metabolism of thyroid hormone is directly related to the inhibition of cellular uptake. These findings underline the potential importance of the plasma membrane in the regulation of thyroid hormone metabolism and, therefore, determination of expression of thyroid hormone activity.
我们研究了载体介导的甲状腺激素转运进入大鼠肝细胞的过程是否是甲状腺激素总细胞摄取和代谢的限速环节。将原代单层培养的大鼠肝细胞在平衡条件下与示踪剂四碘甲状腺原氨酸（T4）、三碘甲状腺原氨酸（T3）或反三碘甲状腺原氨酸（rT3）一起孵育，同时存在或不存在甲状腺激素摄取抑制剂，即哇巴因和抗大鼠肝细胞质膜的单克隆抗体ER-22。所有三种碘甲状腺原氨酸的结果表明，孵育期间培养基中清除率的抑制与碘生成的类似减少平行。这表明甲状腺激素代谢的减少与细胞摄取的抑制直接相关。这些发现强调了质膜在调节甲状腺激素代谢以及因此在确定甲状腺激素活性表达方面的潜在重要性。
