Anesthesiology and Intensive Care Medicine, Department of Surgical and Perioperative Sciences, Umeå University, 90185 Umeå, Sweden.
Department of Chemistry, Umeå University, 901 87 Umeå, Sweden.
Prostaglandins Other Lipid Mediat. 2022 Jun;160:106636. doi: 10.1016/j.prostaglandins.2022.106636. Epub 2022 Mar 17.
Inflammatory signaling pathways involving eicosanoids and other regulatory lipid mediators are a subject of intensive study, and a role for these in acute lung injury is not yet well understood. We hypothesized that oxylipin release from lung injury could be detected in bronchoalveolar lavage fluid and in plasma. In a porcine model of surfactant depletion, ventilation with hyperinflation was assessed. Bronchoalveolar lavage and plasma samples were analyzed for 37 different fatty acid metabolites (oxylipins). Over time, hyperinflation altered concentrations of 4 oxylipins in plasma (TXB, PGE, 15-HETE and 11-HETE), and 9 oxylipins in bronchoalveolar lavage fluid (PGF, PGE, PGD, 12,13-DiHOME, 11,12-DiHETrE, 13-HODE, 9-HODE, 15-HETE, 11-HETE). Acute lung injury caused by high tidal volume ventilation in this porcine model was associated with rapid changes in some elements of the oxylipin profile, detectable in lavage fluid, and plasma. These oxylipins may be relevant in the pathogenesis of acute lung injury by hyperinflation.
涉及花生四烯酸和其他调节脂质介质的炎症信号通路是一个深入研究的课题，这些在急性肺损伤中的作用尚不完全清楚。我们假设肺损伤时肺内脂氧素的释放可以在支气管肺泡灌洗液和血浆中检测到。在表面活性剂耗竭的猪模型中，评估了过度充气通气。分析了支气管肺泡灌洗液和血浆样本中的 37 种不同脂肪酸代谢物（脂氧素）。随着时间的推移，过度充气改变了血浆中 4 种脂氧素（TXB、PGE、15-HETE 和 11-HETE）和支气管肺泡灌洗液中 9 种脂氧素（PGF、PGE、PGD、12,13-DiHOME、11,12-DiHETrE、13-HODE、9-HODE、15-HETE、11-HETE）的浓度。高容量通气引起的这种猪模型中的急性肺损伤与脂氧素谱中一些元素的快速变化相关，这些变化可在灌洗液和血浆中检测到。这些脂氧素可能与过度充气引起的急性肺损伤的发病机制有关。
