Jia Jiaoyan, Liu Ge, Zhong Jianfeng, Yan Ran, Song Xun, Zheng Kai, Ren Zhe, He Zhendan, Zhu Qinchang
College of Pharmacy, Shenzhen Technology University, Shenzhen, China.
School of Pharmaceutical Sciences, Shenzhen University, Shenzhen, China.
Front Microbiol. 2022 Mar 4;13:865644. doi: 10.3389/fmicb.2022.865644. eCollection 2022.
Hand foot and mouth disease (HFMD) caused by Enterovirus 71 (EV71) infection is still a major infectious disease threatening children's life and health in the absence of effective antiviral drugs due to its high prevalence and neurovirulence. A study of EV71-specific host response might shed some light on the reason behind its unique epidemiologic features and help to find means to conquer EV71 infection. We reported that host heat shock protein A6 (HSPA6) was induced by EV71 infection and involved infection in both Rhabdomyosarcoma (RD) cells and neurogliocytes. Most importantly, we found that EV71 did not induce the expression of other heat shock proteins HSPA1, HSPA8, and HSPB1 under the same conditions, and other HFMD-associated viruses including CVA16, CVA6, CVA10, and CVB1-3 did not induce the upregulation of HSPA6. In addition, EV71 infection enhanced the cytoplasmic aggregation of HSPA6 and its colocalization with viral capsid protein VP1. These findings suggest that HSPA6 is a potential EV71-specific host factor worthy of further study.
由肠道病毒71型（EV71）感染引起的手足口病（HFMD），由于其高流行率和神经毒性，在缺乏有效抗病毒药物的情况下，仍然是威胁儿童生命健康的主要传染病。对EV71特异性宿主反应的研究可能有助于揭示其独特流行病学特征背后的原因，并有助于找到攻克EV71感染的方法。我们报道，宿主热休克蛋白A6（HSPA6）由EV71感染诱导，并参与其在横纹肌肉瘤（RD）细胞和神经胶质细胞中的感染。最重要的是，我们发现在相同条件下，EV71不会诱导其他热休克蛋白HSPA1、HSPA8和HSPB1的表达，并且包括柯萨奇病毒A16型（CVA16）、柯萨奇病毒A6型（CVA6）、柯萨奇病毒A10型（CVA10）和柯萨奇病毒B1-3型（CVB1-3）在内的其他手足口病相关病毒不会诱导HSPA6的上调。此外，EV71感染增强了HSPA6的细胞质聚集及其与病毒衣壳蛋白VP1的共定位。这些发现表明，HSPA6是一个值得进一步研究的潜在EV71特异性宿主因子。
