运用元蛋白质组学解析儿童牙齿外源性黑色污渍的成分

Deciphering Black Extrinsic Tooth Stain Composition in Children Using Metaproteomics.

作者信息

Hirtz Christophe, Mannaa Atef Mahmoud, Moulis Estelle, Pible Olivier, O'Flynn Robin, Armengaud Jean, Jouffret Virginie, Lemaistre Camille, Dominici Gabriel, Martinez Alex Yahiaoui, Dunyach-Remy Catherine, Tiers Laurent, Lavigne Jean-Philippe, Tramini Paul, Goldsmith Marie-Christine, Lehmann Sylvain, Deville de Périère Dominique, Vialaret Jerome

机构信息

Univ Montpellier, INM, IRMB, INSERM, CHU Montpellier, CNRS, Montpellier 34070, France.

Higher Institute of Engineering and Technology, New Borg AlArab City 21934, Alexandria, Egypt.

出版信息

ACS Omega. 2022 Feb 25;7(10):8258-8267. doi: 10.1021/acsomega.1c04770. eCollection 2022 Mar 15.

DOI:10.1021/acsomega.1c04770

PMID:35309464

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8928488/

Abstract

The present study focuses on the use of a metaproteomic approach to analyze Black Extrinsic Tooth Stains, a specific type of pigmented extrinsic substance. Metaproteomics is a powerful emerging technology that successfully enabled human protein and bacterial identification of this specific dental biofilm using high-resolution tandem mass spectrometry. A total of 1600 bacterial proteins were identified in black stain (BS) samples and 2058 proteins in dental plaque (DP) samples, whereas 607 and 582 human proteins were identified in BS and DP samples, respectively. A large diversity of bacteria genera (142) in BS and DP was identified, showing a high prevalence of Rothia, Kingella, Neisseria, and Pseudopropionibacterium in black stain samples. In this work, the high diversity of the dental microbiota and its proteome is highlighted, including significant differences between black stain and dental plaque samples.

摘要

本研究聚焦于使用宏蛋白质组学方法分析黑色外源性牙渍，这是一种特定类型的色素性外源性物质。宏蛋白质组学是一项强大的新兴技术，它利用高分辨率串联质谱成功地实现了对这种特定牙菌斑中人类蛋白质和细菌的鉴定。在黑色牙渍（BS）样本中总共鉴定出1600种细菌蛋白，在牙菌斑（DP）样本中鉴定出2058种蛋白，而在BS和DP样本中分别鉴定出607种和582种人类蛋白。在BS和DP中鉴定出了种类繁多的细菌属（142种），显示出黑色牙渍样本中罗氏菌属、金氏菌属、奈瑟菌属和假丙酸杆菌属的高流行率。在这项工作中，突出了牙微生物群及其蛋白质组的高度多样性，包括黑色牙渍和牙菌斑样本之间的显著差异。