双环霉素诱导的大肠杆菌肽聚糖结构修饰

Structural modification of Escherichia coli peptidoglycan induced by bicyclomycin.

作者信息

Pisabarro A G, Cañada F J, Vázquez D, Arriaga P, Rodríguez-Tébar A

出版信息

J Antibiot (Tokyo). 1986 Jul;39(7):914-21. doi: 10.7164/antibiotics.39.914.

DOI:10.7164/antibiotics.39.914

Abstract

We have studied the modification of Escherichia coli peptidoglycan induced by bicyclomycin. For this purpose liquid chromatography for peptidoglycan analysis has been used. The main alteration found was an increase of diaminopimelyl-diaminopimelyl bridge containing subunits. Our results show that bicyclomycin impairs the normal breakage of that interpeptidic bond, whose cleavage is needed for the normal remodeling of peptidoglycan and cell growth. Based on the analysis of the possible structure of diaminopimelyl-diaminopimelyl bond and bicyclomycin, we propose a hypothesis on the mechanism of action of bicyclomycin.

摘要

我们研究了双环霉素对大肠杆菌肽聚糖的修饰作用。为此，采用了用于肽聚糖分析的液相色谱法。发现的主要变化是含二氨基庚二酰 - 二氨基庚二酰桥的亚基增加。我们的结果表明，双环霉素损害了该肽间键的正常断裂，而该键的断裂是肽聚糖正常重塑和细胞生长所必需的。基于对二氨基庚二酰 - 二氨基庚二酰键和双环霉素可能结构的分析，我们提出了双环霉素作用机制的假说。