Department of Gynecology, Renmin Hospital of Wuhan University, Wuhan, China.
Department of Obstetrics and Gynecology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China.
Analyst. 2022 Apr 11;147(8):1534-1539. doi: 10.1039/d1an02003j.
Single base mutations are closely related to cancer diagnosis and treatment. The fluorescent probe method is one of the important methods to detect single-base mutations. We constructed a universal probe detection system based on endonuclease IV and the DNA strand displacement reaction. The system uses two toehold strand displacement reactions to relay the mutation information to the universal strand. There is no need to design the probe one-by-one for each mutation point during multi-site detection. It has the advantages of simple operation, rapid detection, and low cost. We used this method to detect common clinical mutation sites (PTEN R130Q/EGFR L858R/PTEN rs1473918395), and the detection limit can reach 0.1%-1%. The detection system can provide a new rapid and economical method for clinical single-base mutation detection, and has broad application prospects in diagnosis and prognostic evaluation.
单碱基突变与癌症的诊断和治疗密切相关。荧光探针法是检测单碱基突变的重要方法之一。我们构建了基于内切酶 IV 和 DNA 链置换反应的通用探针检测系统。该系统使用两个引发链置换反应将突变信息中继到通用链上。在多靶点检测中，无需为每个突变点逐一设计探针。它具有操作简单、检测快速、成本低的优点。我们使用该方法检测了常见的临床突变位点（PTEN R130Q/EGFR L858R/PTEN rs1473918395），检测限可达 0.1%-1%。该检测系统可为临床单碱基突变检测提供一种快速、经济的新方法，在诊断和预后评估方面具有广阔的应用前景。
