Morin O, Normand C
J Cell Physiol. 1986 Oct;129(1):103-10. doi: 10.1002/jcp.1041290115.
Sinusoidal cells isolated from adult rat liver have been established in primary culture and in cell line. The presence of factor VIII R:Ag and peroxidatic/phagocytosis activities were the criteria used to distinguish in freshly isolated cells the endothelial cells from the Kupffer cells and suggested the endothelial origin of the cell line. Using a co-culture system, the effect of sinusoidal liver cells on hepatocyte functional activity was characterized. A plateau in which the state of differentiation was stabilized could be generated for co-cultured hepatocytes isolated from adult rat and a disappearance of the initial expression of alpha 1-fetoprotein (AFP) and the increase and/or maintenance of albumin secretion were measured with co-cultured hepatocytes isolated from suckling rat. The presence of dexamethasone was required for such beneficial effect. The hepatocyte-stabilizing activity was also produced by a pulmonary endothelial cell line.
从成年大鼠肝脏分离出的窦状细胞已在原代培养和细胞系中建立。因子VIII R:Ag的存在以及过氧化物酶/吞噬活性是用于在新鲜分离的细胞中区分内皮细胞和库普弗细胞的标准，并提示了该细胞系的内皮起源。使用共培养系统，对肝窦状细胞对肝细胞功能活性的影响进行了表征。对于从成年大鼠分离的共培养肝细胞，可以产生一个分化状态得以稳定的平台期，并且对于从乳鼠分离的共培养肝细胞，可检测到α1-甲胎蛋白（AFP）初始表达的消失以及白蛋白分泌的增加和/或维持。这种有益作用需要地塞米松的存在。肺内皮细胞系也产生肝细胞稳定活性。
