School of Automation Engineering, University of Electronic Science and Technology of China, Chengdu, P. R. China.
Department of Electronic, Electrical, and Systems Engineering, University of Birmingham, Birmingham, UK.
Electrophoresis. 2022 Nov;43(21-22):2250-2259. doi: 10.1002/elps.202100359. Epub 2022 May 6.
As the medical community puts forward higher requirements for the speed and convenience of disease diagnosis, point-of-care testing has become a hot research topic to overcome various kinds of healthcare problems. Blood test is considered to be highly sensitive and accurate in clinical diagnosis. However, conventional plasma separation system tends to be bulky and needs professional operations. Moreover, imprecise separation may cause residual biochemical substances such as blood cells to affect the detection results. In this work, to solve these problems, we designed a portable centrifugal microfluidic platform for automatic, rapid and ultraprecise blood separation. The disc consists of multichambers and multi-microchannels where a plasma reservoir and a cell reservoir are connected to each other and collinear with the center of the circle. This structure overcomes the weakness of low separation efficiency (when hematocrit increases) under the traditional blood separation structure (bifurcation structure). As a result, the proposed system achieved 99.9% plasma purity, 99.9% separation efficiency (with a blood hematocrit of 48%) and 32.5% plasma recovery rate in the 50s, which provides a strong guarantee for rapid blood diagnosis and analysis, especially in areas where medical resources are limited.
随着医学界对疾病诊断速度和便利性提出了更高的要求，即时检测已成为克服各种医疗保健问题的热门研究课题。血液检测在临床诊断中被认为具有高度的敏感性和准确性。然而，传统的血浆分离系统往往体积庞大，需要专业操作。此外，不精确的分离可能会导致残留的生化物质如血细胞影响检测结果。在这项工作中，为了解决这些问题，我们设计了一种用于自动、快速和超精确血液分离的便携式离心微流控平台。该圆盘由多个腔室和微通道组成，其中血浆储液器和细胞储液器彼此相连并与圆盘的中心共线。这种结构克服了传统血液分离结构（分叉结构）下分离效率低（当血细胞比容增加时）的弱点。因此，所提出的系统在 50 秒内实现了 99.9%的血浆纯度、99.9%的分离效率（血细胞比容为 48%）和 32.5%的血浆回收率，为快速血液诊断和分析提供了有力保障，特别是在医疗资源有限的地区。
