van Duinen S G, Ruitter D J, Ferrone S
J Pathol. 1986 Aug;149(4):339-48. doi: 10.1002/path.1711490410.
Malignant transformation of melanocytes and further neoplastic progression may be associated with qualitative and/or quantitative changes in expression of HLA class I and class II antigens. Since previous immunohistochemical studies of surgically removed melanoma lesions have suggested a relationship in the expression of HLA class I and class II antigens, we have investigated the expression of these antigens at the single cell level. Double immunofluorescence staining of frozen sections of melanoma metastases and immunoelectron microscopic double labelling of melanoma cell suspensions prepared from three of these lesions has detected three HLA phenotypes on the large majority of melanoma cells: either both HLA class I and class II antigens, neither HLA antigen or only HLA class I antigens. In four out of the 11 lesions a few melanoma cells were found to express HLA class II antigens and to lack HLA class I antigens. A relationship was also found in the level of expression of HLA class I and class II antigens, as estimated by the intensity of staining with monoclonal antibodies. The level of expression of HLA class II antigens appeared to be similar to or lower than that of HLA class I antigens on the large majority of melanoma cells. This coordinated heterogeneity in the expression of HLA class I and class II antigens by melanoma cells may have implications in the interactions of tumour cells with the host's immune system.
黑素细胞的恶性转化及进一步的肿瘤进展可能与HLA I类和II类抗原表达的质和/或量的变化有关。由于先前对手术切除的黑色素瘤病变进行的免疫组织化学研究表明HLA I类和II类抗原的表达之间存在关联，我们在单细胞水平上研究了这些抗原的表达。对黑色素瘤转移灶冰冻切片进行双重免疫荧光染色，并对其中三个病变制备的黑色素瘤细胞悬液进行免疫电镜双重标记，结果在绝大多数黑色素瘤细胞上检测到三种HLA表型：HLA I类和II类抗原均表达、两种HLA抗原均不表达或仅表达HLA I类抗原。在11个病变中的4个病变中，发现少数黑色素瘤细胞表达HLA II类抗原而缺乏HLA I类抗原。通过单克隆抗体染色强度估计，还发现了HLA I类和II类抗原表达水平之间的关联。在绝大多数黑色素瘤细胞上，HLA II类抗原的表达水平似乎与HLA I类抗原相似或低于HLA I类抗原。黑色素瘤细胞HLA I类和II类抗原表达的这种协调性异质性可能对肿瘤细胞与宿主免疫系统的相互作用有影响。
