Department of Pathology, Microbiology, & Immunology, Vanderbilt University Medical Center, Nashville, TN, USA.
Vanderbilt Institute for Infection, Immunology, and Inflammation, Vanderbilt University, Nashville, TN, USA.
Nat Commun. 2022 Mar 21;13(1):1491. doi: 10.1038/s41467-022-29209-4.
HitRS is a two-component system that responds to cell envelope damage in the human pathogen Bacillus anthracis. Here we identify an RNA-binding protein, KrrA, that regulates HitRS function by modulating the stability of the hitRS mRNA. In addition to hitRS, KrrA binds to over 70 RNAs and, directly or indirectly, affects the expression of over 150 genes involved in multiple processes, including genetic competence, sporulation, RNA turnover, DNA repair, transport, and cellular metabolism. KrrA does not exhibit detectable nuclease activity in vitro, and thus the mechanism by which it modulates mRNA stability remains unclear.
HitRS 是一个由两个部分组成的系统，它可以响应人类病原体炭疽杆菌的细胞包膜损伤。在这里，我们发现了一种 RNA 结合蛋白 KrrA，它可以通过调节 hitRS mRNA 的稳定性来调节 HitRS 的功能。除了 hitRS，KrrA 还与 70 多个 RNA 结合，并直接或间接地影响超过 150 个基因的表达，这些基因涉及多个过程，包括遗传能力、孢子形成、RNA 周转、DNA 修复、运输和细胞代谢。KrrA 在体外没有表现出可检测的核酸酶活性，因此它调节 mRNA 稳定性的机制尚不清楚。
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