ProFlow nano-liquid chromatography with a graphene oxide-functionalized monolithic nano-column for the simultaneous determination of chloramphenicol and chloramphenicol glucuronide in foods.

Chloramphenicol (CAP) is an effective antibiotic with broad spectrum against gram-positive and gram-negative bacteria, while it is used to treat various infections in animals. Although CAP is banned for usage in the livestock products including, milk, honey, seafood, and royal jelly, CAP is still often detected in foods of animal origin, posing a threat to consumer health. The use of CAP is restricted in many countries due to its side effect in human metabolic process according to the Expert Committee on Food Additives (ECFA) recommendation. Chloramphenicol glucuronide (CAPG) is also a metabolic product of CAP, which may be a hazardous chemical for human health. Therefore, the development of sensitive separation and quantification method is an important issue, especially for food safety. Herein, we reported the preparation and application of a monolithic nano-column for CAP and CAPG analyses in foods by ProFlow Nano liquid chromatography (ProFlow Nano LC). The monolithic nano-column was prepared by an in situ polymerization using 3-chloro-2-hydroxypropylmethacrylate (HPMA-Cl) and ethylene dimethacrylate (EDMA) and followed graphene oxide (GO) modification. After characterization, the monolithic nano-column was used for the analysis of CAP and CAPG in honey and milk samples by ProFlow Nano LC. The whole method was validated in terms of linearity, sensitivity, precision, recovery, and repeatability, while it led to obtain high sensitivity with limit of quantification was found as 0.02 µg/kg for CAP. Limit of quantification for CAPG was found as 0.08 µg/kg. The developed method with monolithic nano-column was optimized to achieve very sensitive analyses of CAP and CAPG in the food samples. The applicability of the nano-column was successfully demonstrated by the analysis of CAP and CAPG in milk and honey samples. PRACTICAL APPLICATION: This article describes the preparation and application of a monolithic nano-column for the separation and determination of chloramphenicol and chloramphenicol glucuronide in food samples by ProFlow Nano LC. The use of new and advanced techniques is a crucial issue in the food science and technology. In this sense, this study demonstrated a new food analysis method using advanced instrumental technique with a homemade monolithic nano-column.