Tran Hanh My, Nguyen Dung Thuy Thi, Mai Ngan Thi, Do Hien Thi, Nguyen Thanh Kim Thi, Nguyen Thao Kim Nu, Muller Marc, Nguyen Huy Quang, Pham Hai The
Department of Microbiology, Faculty of Biology, VNU University of Science - Vietnam National Universiy, 334 Nguyen Trai, Thanh Xuan, Hanoi, Vietnam.
Department of Life Sciences, GIGA-I3, Laboratory for Organogenesis and Regeneration, University of Liege, 4000, Liège 1, Belgium.
Curr Microbiol. 2022 Mar 22;79(5):140. doi: 10.1007/s00284-022-02830-8.
Endophytes can generate a cornucopia of marvelous bioactive secondary metabolites useful for mankind but their biodiversity and associations with host plants are still elusive. In this study, we explored the culturable endophytic microorganisms associated with 14 medicinal plants that are of high socio-economic value and/or reportedly endemic to northern Vietnam. Specifically, we isolated the endophytic microorganisms by applying surface sterilization methods and identified them based on morphological and rDNA sequence analyses. Agglomerative Hierarchical Clustering (AHC) and Principal Component Analysis (PCA) were used to analyze the correlations between the taxonomic affiliations of the culturable endophytes and the characteristics of their hosts. Most of the culturable endophytes obtained were bacteria (80), and few of those were actinomycetes (15) and fungi (8). Many of them are reported to be endophytes of medicinal plants for the first time. A number of plants (5) are also reported for the first time to contain microbial endophytes, while some plants with powerful pharmaceutical potential harbor unique endophytes. Furthermore, our results reveal a strikingly close relation between the compositions of bacterial and fungal isolates from plants having anti-bacterial activity and those from plants having anti-inflammatory activity, or between the compositions of the microbial endophytic isolates from plants having anti-cancer activity and those from plants having antioxidant activity. Altogether, the results provide new findings which can be inspiring for further in-depth studies to explore and exploit the relationships between medicinal plants and their associated endophytes in northern Vietnam and world-wide.
内生菌能够产生大量对人类有益的奇妙生物活性次生代谢产物，但其生物多样性以及与宿主植物的关联仍不明确。在本研究中，我们探索了与14种具有高社会经济价值和/或据报道为越南北部特有的药用植物相关的可培养内生微生物。具体而言，我们通过应用表面灭菌方法分离内生微生物，并基于形态学和rDNA序列分析对其进行鉴定。使用凝聚层次聚类（AHC）和主成分分析（PCA）来分析可培养内生菌的分类归属与其宿主特征之间的相关性。获得的大多数可培养内生菌是细菌（80种），其中少数是放线菌（15种）和真菌（8种）。据报道，它们中的许多是首次作为药用植物的内生菌。一些植物（5种）也首次被报道含有微生物内生菌，而一些具有强大药用潜力的植物含有独特的内生菌。此外，我们的结果揭示了具有抗菌活性的植物的细菌和真菌分离物组成与具有抗炎活性的植物的细菌和真菌分离物组成之间，或者具有抗癌活性的植物的微生物内生分离物组成与具有抗氧化活性的植物的微生物内生分离物组成之间存在着惊人的密切关系。总之，这些结果提供了新的发现，可为进一步深入研究以探索和利用越南北部及全球药用植物与其相关内生菌之间的关系提供启发。
