Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland.
Institute of Chemical Technology and Engineering, Faculty of Chemical Technology, Poznan University of Technology, Berdychowo 4, PL-60965 Poznan, Poland; Process and Systems Engineering Centre (PROSYS), Department of Chemical and Biochemical Engineering, Technical University of Denmark, Søltofts Plads, Building 227, DK-2800 Kongens Lyngby, Denmark.
J Hazard Mater. 2022 Jun 15;432:128688. doi: 10.1016/j.jhazmat.2022.128688. Epub 2022 Mar 16.
The presence of micropollutants in water, wastewater and soil are a global problem due to their persistent effect on ecosystems and human health. Although there are many methods of removal of environmental pollutants, they are often ineffective for degradation of pharmaceuticals, including estrogens. In presented work we proposed fabrication of electrospun material from polyacrylonitrile/polyethersulfone (PAN/PES) as a support for laccase immobilization by covalent binding. Oxidoreductase was attached to the electrospun fibers using polydopamine as a linker and produced system was used for degradation of two estrogens: 17β-estradiol (E2) and 17α-ethynylestradiol (EE2). It was shown that 92% of E2 and 100% of EE2 were degraded after 24 h of the process. Moreover, the effect of surfactants, metal ions and mediators on conversion efficiencies of estrogens was investigated and it was confirmed that immobilized enzyme possessed higher resistance to inhibitory agents as well as thermal and storage stability, compared to its native form. Finally, estrogenic activities of E2 and EE2 solutions decreased around 99% and 87%, respectively, after enzymatic conversion, that corresponds to significant reduction of the total organic carbon and formation of low-toxic final products of estrogens degradation.
水中、废水中和土壤中存在的微污染物是一个全球性问题，因为它们对生态系统和人类健康具有持久的影响。尽管有许多去除环境污染物的方法，但它们通常无法有效降解药物，包括雌激素。在本工作中，我们提出了一种由聚丙烯腈/聚醚砜（PAN/PES）制成的电纺材料的制备方法，作为通过共价键合固定漆酶的载体。使用聚多巴胺作为连接物将氧化还原酶连接到电纺纤维上，并使用该系统降解两种雌激素：17β-雌二醇（E2）和 17α-乙炔基雌二醇（EE2）。结果表明，在 24 小时的处理过程中，E2 的降解率达到 92%，EE2 的降解率达到 100%。此外，还研究了表面活性剂、金属离子和介体对雌激素转化率的影响，结果证实固定化酶对抑制剂以及热和储存稳定性具有更高的抗性，与天然形式相比。最后，在酶转化后，E2 和 EE2 溶液的雌激素活性分别降低了约 99%和 87%，这对应于总有机碳的显著减少和雌激素降解的低毒终产物的形成。
