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长链非编码RNA TP73-AS1预测宫颈癌预后不良并调控其细胞增殖和迁移

Long non-coding RNA TP73-AS1 predicts poor prognosis and regulates cell proliferation and migration in cervical cancer.

作者信息

Song Zhijiao, Xing Feng, Jiang Huici, He Yuanying, Lv Jia

机构信息

Department of Obstetrics and Gynecology, Shanghai Tenth People's Hospital, Tongji University, Shanghai, China.

出版信息

Arch Med Sci. 2019 Sep 5;18(2):523-534. doi: 10.5114/aoms.2019.87686. eCollection 2022.

DOI:10.5114/aoms.2019.87686
PMID:35316908
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8924823/
Abstract

INTRODUCTION

Cervical cancer is one of the most common malignant tumors in women, which seriously affects women's health, especially in developing countries. This study aims to investigate novel molecular markers for poor prognosis of cervical cancer to achieve correct guidance of clinical treatment, accurate assessment of prognosis, and provide a new basis for the choice of reasonable treatment options for cervical cancer patients.

MATERIAL AND METHODS

QRT-PCR was employed to investigate the expression of lncRNA TP73-AS1 in cervical cancer tissues and cell lines. COX multivariate analysis showed the relationship between TP73-AS1 expression and clinicopathological features of patients with cervical cancer. Colony formation and MTT assay detected the effect of TP73-AS1 on proliferation of cervical cancer cells. The effect of TP73-AS1 on migration and invasion of cervical cancer cells was determined by the wound-healing assay and transwell assay. Western blot was performed to assess the expression of EMT markers.

RESULTS

This study showed that lncRNA TP73-AS1 was up-regulated in cervical cancer tissues and cell lines ( < 0.001), and high expression of TP73-AS1 could be considered as an independent prognostic factor ( < 0.05). Moreover, lncRNA TP73-AS1 promotes cervical cancer cell migration and invasion, and knockdown of TP73-AS1 inhibits the growth of cervical cancer cells ( < 0.001).

CONCLUSIONS

Our results indicated that lncRNA TP73-AS1 was up-regulated in cervical cancer tissues and cell lines, predicting poor prognosis of cervical cancer and regulating cell proliferation and migration.

摘要

引言

宫颈癌是女性最常见的恶性肿瘤之一，严重影响女性健康，在发展中国家尤为如此。本研究旨在探究宫颈癌预后不良的新型分子标志物，以实现对临床治疗的正确指导、准确评估预后，并为宫颈癌患者合理治疗方案的选择提供新依据。

材料与方法

采用定量逆转录聚合酶链反应（QRT-PCR）检测lncRNA TP73-AS1在宫颈癌组织和细胞系中的表达。COX多因素分析显示TP73-AS1表达与宫颈癌患者临床病理特征之间的关系。集落形成实验和MTT实验检测TP73-AS1对宫颈癌细胞增殖的影响。通过划痕实验和Transwell实验确定TP73-AS1对宫颈癌细胞迁移和侵袭的影响。采用蛋白质免疫印迹法检测上皮-间质转化（EMT）标志物的表达。

结果

本研究表明，lncRNA TP73-AS1在宫颈癌组织和细胞系中上调（<0.001），TP73-AS1高表达可被视为独立预后因素（<0.05）。此外，lncRNA TP73-AS1促进宫颈癌细胞迁移和侵袭，敲低TP73-AS1可抑制宫颈癌细胞生长（<0.001）。

结论

我们的结果表明，lncRNA TP73-AS1在宫颈癌组织和细胞系中上调，预示宫颈癌预后不良，并调节细胞增殖和迁移。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/987e/8924823/ed4e56d080a9/AMS-18-2-110729-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/987e/8924823/e3d273110fc5/AMS-18-2-110729-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/987e/8924823/05d14fe0d27b/AMS-18-2-110729-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/987e/8924823/079e268a39ed/AMS-18-2-110729-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/987e/8924823/ed4e56d080a9/AMS-18-2-110729-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/987e/8924823/e3d273110fc5/AMS-18-2-110729-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/987e/8924823/05d14fe0d27b/AMS-18-2-110729-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/987e/8924823/079e268a39ed/AMS-18-2-110729-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/987e/8924823/ed4e56d080a9/AMS-18-2-110729-g004.jpg

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