Hernández F, Vioque A
Mol Biol Rep. 1986;11(3):137-41. doi: 10.1007/BF00419733.
Modification of 40S ribosomal subunits from Saccharomyces cerevisiae with dimethylmaleic anhydride (DMMA), a reagent for protein amino groups, is accompanied by loss of polypeptide-synthesizing activity and by dissociation of proteins from the particles. The protein-deficient ribosomal particles, originated from 40S subunits by treatment with dimethylmaleic anhydride at a molar ratio of reagent to particle of 250, can partially reconstitute active subunits upon addition of the corresponding released proteins, and regeneration of the modified amino groups.
用蛋白质氨基试剂顺丁烯二酸二甲酯（DMMA）修饰酿酒酵母的40S核糖体亚基，会伴随着多肽合成活性的丧失以及蛋白质从颗粒上的解离。当试剂与颗粒的摩尔比为250时，用顺丁烯二酸二甲酯处理40S亚基产生的蛋白质缺陷型核糖体颗粒，在添加相应释放的蛋白质并使修饰的氨基再生后，能够部分重构活性亚基。
