依托度酸增强了辐照 HT-29 人结肠癌细胞的放射敏感性。
Etodolac Enhances the Radiosensitivity of Irradiated HT-29 Human Colorectal Cancer Cells.
机构信息
Research Center for Molecular Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Nuclear Medicine and Molecular Imaging, Clinical Development Research Unit of Farshchian Heart Center, Hamadan University of Medical Sciences, Hamadan, Iran.
出版信息
Curr Radiopharm. 2022;15(3):242-248. doi: 10.2174/1874471015666220321143139.
BACKGROUND
Radioresistance is found to be the main therapeutic restriction in colorectal radiation therapy. The aim of this study was to investigate the synergistic effect of Etodolac (ET) and ionizing radiation on human colorectal cancer cells.
METHODS
Pretreated HT-29 cells with ET were exposed to ionizing radiation. The radiosensitizing effect of ET was evaluated using MTT, flow cytometry, and clonogenic assay. The amount of nitrite oxide (NO) in irradiated cells was also measured with the Griess reagent.
RESULTS
The present study found that pretreatment of HT-29 cells with ET decreases their survival and colony formation. Higher concentrations of ET cause total apoptosis and an increase in NO levels in irradiated cells.
CONCLUSION
Applying ET in a concentration-dependent manner had an incremental effect on the amount of apoptosis and cell death induced by radiation.
背景
放射抵抗被认为是结直肠放射治疗的主要治疗限制。本研究旨在探讨依托度酸（ET）与电离辐射对人结直肠癌细胞的协同作用。
方法
用 ET 预处理 HT-29 细胞后进行电离辐射。用 MTT、流式细胞术和集落形成试验评估 ET 的放射增敏作用。用格里试剂测量照射细胞中亚硝酸盐（NO）的含量。
结果
本研究发现，ET 预处理 HT-29 细胞可降低其存活率和集落形成率。更高浓度的 ET 导致照射细胞中的总凋亡和 NO 水平增加。
结论
以浓度依赖的方式应用 ET 可增加辐射诱导的细胞凋亡和死亡的数量。
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