State Key Laboratory of Environmental Chemistry and Ecotoxicology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
University of Chinese Academy of Sciences, Beijing, 100049, China.
Anal Methods. 2022 Apr 7;14(14):1430-1438. doi: 10.1039/d2ay00029f.
Halogenated polycyclic aromatic hydrocarbons (HPAHs) have attracted extensive attention because of their high toxicity and bioaccumulation. However, there has been no report on the content of HPAHs in human tissues and the corresponding analytical method. In this study, a method for the simultaneous determination of 16 polycyclic aromatic hydrocarbons (PAHs) and 23 HPAHs in human serum was developed and validated. Simple and stable removal of interfering substances in complex serum and the detection of ultra-trace HPAHs are the key difficulties. After 0.5 mL serum was treated with formic acid and 10% isopropanol, samples were prepared by solid phase extraction (SPE) and analyzed by gas chromatography-triple quadrupole mass spectrometry (GC-MS/MS). The recoveries of the method were 65-103%, with low detection limits of 0.001-0.019 ng mL. For HPAHs, the precision was in the range of 0.2-10% according to relative standard deviation (RSD). Subsequently, the developed method was validated for serum samples obtained in hospitals, and 8 PAHs and 12 HPAHs were detected. The concentration of ∑HPAHs was 23 ± 12 ng g lipid in females and 21 ± 10 ng g lipid in males, in which phenanthrene and anthracene halogenated derivatives were the main components. The level of HPAHs was correlated with PAHs, which was 23-119 times higher than that of HPAHs. The detected HPAHs contain highly toxic and persistent components, representing an ongoing human health risk, which should receive more attention.
卤代多环芳烃（HPAHs）因其高毒性和生物累积性而受到广泛关注。然而，目前还没有关于人类组织中 HPAHs 含量及其相应分析方法的报道。本研究建立并验证了一种同时测定人血清中 16 种多环芳烃（PAHs）和 23 种 HPAHs 的方法。复杂血清中干扰物质的简单稳定去除和痕量 HPAHs 的检测是关键难点。用甲酸和 10%异丙醇处理 0.5mL 血清后，通过固相萃取（SPE）进行样品制备，并用气相色谱-三重四极杆质谱（GC-MS/MS）进行分析。该方法的回收率为 65-103%，检测限低至 0.001-0.019ng mL。对于 HPAHs，根据相对标准偏差（RSD），精密度在 0.2-10%范围内。随后，对医院获得的血清样本进行了方法验证，并检测到 8 种 PAHs 和 12 种 HPAHs。∑HPAHs 在女性中的浓度为 23±12ng g 脂质，在男性中的浓度为 21±10ng g 脂质，其中菲和蒽的卤代衍生物为主要成分。HPAHs 的水平与 PAHs 相关，其浓度是 HPAHs 的 23-119 倍。检测到的 HPAHs 含有高毒性和持久性成分，代表着持续存在的人类健康风险，应引起更多关注。
