Institute of Biomedical Sciences, Academia Sinica, Taipei, Taiwan.
Methods Mol Biol. 2022;2442:353-365. doi: 10.1007/978-1-0716-2055-7_19.
Galectins are animal lectins that recognize β-galactoside and bind glycans. Recent studies have indicated that cytosolic galectins recognize cytosolically exposed glycans and accumulate around endocytic vesicles or organelles damaged by various disruptive substances. Accumulated galectins engage other cytosolic proteins toward damaged vesicles, leading to cellular responses, such as autophagy. Disruptive substances include bacteria, viruses, particulate matters, and protein aggregates; thus, this process is implicated in the pathogenesis of various diseases. In this chapter, we describe methods for studying three disruptive substances: photosensitizers, Listeria monocytogenes, and Helicobacter pylori. We summarize the tools used for the detection of cytosolic galectin accumulation around damaged vesicles.
半乳糖凝集素是识别β-半乳糖苷并结合糖链的动物凝集素。最近的研究表明，细胞质半乳糖凝集素识别细胞质中暴露的糖链，并在各种破坏物质损伤的内吞小泡或细胞器周围积累。积累的半乳糖凝集素将其他细胞质蛋白募集到受损的小泡上，从而引发细胞反应，如自噬。破坏物质包括细菌、病毒、颗粒物质和蛋白质聚集体；因此，这个过程与各种疾病的发病机制有关。在本章中，我们描述了研究三种破坏物质的方法：光敏剂、李斯特菌和幽门螺杆菌。我们总结了用于检测受损小泡周围细胞质半乳糖凝集素积累的工具。
