Medical Academy named after S.I. Georgievsky of Vernadsky CFU.
AO "Vector-Best".
Klin Lab Diagn. 2022 Mar 25;67(3):170-176. doi: 10.51620/0869-2084-2022-67-3-170-176.
The paper presents the results of a study of the prevalence of Ixodid ticks - potential carriers of tick-borne rickettsiosis pathogens. Ectoparasites were collected in various natural and climatic zones of the Crimean Peninsula within the year 2016-2018. As a result of screening with the help of real-time PCR analysis (PCR-RT), a genetic marker (a section of the gltA gene) of the rickettsia group of tick-borne spotted fever was detected in ticks. The most common DNA marker of rickettsia was found in ticks in the eastern regions of the steppe zone - 50,6 %, in the north-western part of the steppe zone this value was 12,0 %. The least amount of rickettsia target DNA was detected in ticks collected in the mountain forest and south bank zones - 4,5 %. As a result of sequencing of positive DNA samples from fragments of the gltA, ompA, ompB, and sca4 genes, the species composition of rickettsias was established. The DNA of 8 species of rickettsia was identified: Circulation of three R. conorii, R. massiliae, R. sibirica subsp. mongolotimonae, R. slovaca, R. aeschlimannii, R. monacensis, R. helvetica, R. raoultii. R. massiliae, R. slovaca, and R. helvetica were established in the Crimean Peninsula for the first time. The peculiarities of the geographical distribution of the identified rickettsia species were determined, which was due to the spread of mites-carriers of pathogens. The revealed diversity of rickettsia species and their vectors, due to the isolation of the areas of the main feeding animals and the established routes of migratory birds, suggests the circulation of other rickettsia species on the territory of the Crimean Peninsula. The obtained results suggest that the diseases of tick-borne rickettsiosis in the Crimean Peninsula can be caused not only by R. conorii, as previously thought, but also by other types of rickettsii.
本文介绍了一项关于硬蜱——蜱传斑点热病原体潜在携带者——流行情况的研究结果。在 2016 年至 2018 年期间，在克里米亚半岛的各种自然和气候带中采集了这些外寄生虫。通过实时 PCR 分析（PCR-RT）的帮助进行筛选后，在蜱中检测到了蜱传斑点热立克次体群的遗传标记（gltA 基因的一个片段）。在草原带的东部地区，最常见的蜱携带立克次体 DNA 标记为 50.6%，在草原带的西北部分，这一数值为 12.0%。在山地森林和南岸带采集的蜱中，检测到的立克次体靶 DNA 最少，为 4.5%。对 gltA、ompA、ompB 和 sca4 基因片段的阳性 DNA 样本进行测序后，确定了立克次体的种组成。鉴定出 8 种立克次体的 DNA：循环型三种、R. massiliae、R. sibirica subsp. mongolotimonae、R. slovaca、R. aeschlimannii、R. monacensis、R. helvetica、R. raoultii。首次在克里米亚半岛发现了 R. massiliae、R. slovaca 和 R. helvetica。确定了所鉴定的立克次体种的地理分布特点，这是由于病原体传播的螨虫载体的扩散所致。由于主要食源动物的隔离以及候鸟迁徙路线的建立，所发现的立克次体种多样性及其载体表明，在克里米亚半岛的领土上可能还存在其他立克次体种的循环。研究结果表明，克里米亚半岛的蜱传立克次体病不仅可以由以前认为的 R. conorii 引起，还可以由其他类型的立克次体引起。
