[Aspartase activity of Escherichia coli, strain 85, cells grown on glucose-mineral medium with yeast extract].

The conditions for cultivation of E. coli 85 on a glucose-mineral medium with a yeast extract were optimized. The cells obtained had a high aspartate ammonia-lyase activity. The aspartase activity was determined kinetically both by consumption of the substrate, ammonium fumarate, and by accumulation of the product, aspartic acid.