Department of Urology, Second Hospital of Jilin University, China.
Department of Urology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea.
Oxid Med Cell Longev. 2022 Mar 14;2022:5213573. doi: 10.1155/2022/5213573. eCollection 2022.
Low-intensity extracorporeal shockwave therapy (Li-ESWT), as a microenergy therapy, has the effects of inhibiting oxidative stress, antiapoptosis, and tissue repair, which is increasingly applied to a variety of diseases. Our research aims to explore the protective effects of Li-ESWT in the aging rat model and its possible molecular mechanism through in vivo and in vitro experiments. In vitro, TM3 Leydig cells incubated with HO were treated with Li-ESWT at 4 energy levels (0.01, 0.05, 0.1, and 0.2 mJ/mm). In vivo, we employed an androgen-deficient rat model to simulate male aging and treated it with Li-ESWT at three different energy levels (0.01, 0.05, and 0.2 mJ/mm). Li-ESWT increased the expression of vascular endothelial growth factor (VEGF) in TM3 cells, improved antioxidant capacity, and reduced apoptosis, with the effect being most significant at 0.05 mJ/mm energy level. In androgen-deficient rat model, LI-ESWT can improve sperm count, motility, and serum testosterone level, enhancing tissue antioxidant capacity and antiapoptotic ability, and the effect is most significant at 0.05 mJ/mm energy level. Therefore, Li-ESWT at an appropriate energy level can improve sperm count, motility, and serum testosterone levels in androgen-deficient rat models, reduce oxidative stress in the testis, and increase antioxidant capacity and antiapoptotic abilities. The mechanism of this condition might be related to the increased VEGF expression in Leydig cells by Li-ESWT.
低强度体外冲击波疗法（Li-ESWT）作为一种微能量疗法，具有抑制氧化应激、抗细胞凋亡和组织修复的作用，越来越多地应用于多种疾病。我们的研究旨在通过体内和体外实验探索 Li-ESWT 在衰老大鼠模型中的保护作用及其可能的分子机制。在体外，HO 孵育的 TM3 莱迪希细胞用 Li-ESWT 在 4 个能量水平（0.01、0.05、0.1 和 0.2 mJ/mm）下处理。在体内，我们采用雄激素缺乏大鼠模型模拟男性衰老，并以 3 种不同能量水平（0.01、0.05 和 0.2 mJ/mm）用 Li-ESWT 进行处理。Li-ESWT 增加了 TM3 细胞中血管内皮生长因子（VEGF）的表达，提高了抗氧化能力，减少了细胞凋亡，在 0.05 mJ/mm 能量水平时效果最为显著。在雄激素缺乏大鼠模型中，Li-ESWT 可以提高精子数量、活力和血清睾酮水平，增强组织抗氧化能力和抗凋亡能力，在 0.05 mJ/mm 能量水平时效果最为显著。因此，Li-ESWT 在适当的能量水平可以提高雄激素缺乏大鼠模型中的精子数量、活力和血清睾酮水平，减少睾丸的氧化应激，并增加抗氧化能力和抗凋亡能力。这种情况的机制可能与 Li-ESWT 增加莱迪希细胞中 VEGF 的表达有关。
