Hainan Key Lab of Tropical Animal Reproduction, Breeding and Epidemic Disease Research, Animal Genetic Engineering Key Lab of Haikou, College of Animal Science and Technology, Hainan University, Haikou, Hainan, China.
PeerJ. 2022 Mar 18;10:e13047. doi: 10.7717/peerj.13047. eCollection 2022.
() is a zoonotic bacterium that predominantly colonizes the respiratory tract and lungs of a variety of farmed and wild animals, and causes severe respiratory disease. To investigate the characteristics of the host immune response induced by strains of serotype A and D, high-throughput mRNA-Seq and miRNA-Seq were performed to analyze the changes in goat bronchial epithelial cells stimulated by these two serotypes of for 4 h. Quantitative RT-PCR was used to validate the randomly selected genes and miRNAs. The results revealed 204 and 117 differentially expressed mRNAs (|log(Fold-change)| ≥ 1, < 0.05) in the serotype A and D stimulated groups, respectively. Meanwhile, the number of differentially expressed miRNAs (|log(Fold-change)| > 0.1, < 0.05) were 269 and 290, respectively. GO and KEGG enrichment analyses revealed 13 GO terms ( < 0.05) and four KEGG pathways ( < 0.05) associated with immunity. In the serotype A-stimulated group, the immune-related pathways were the GABAergic synapse and Toll-like receptor signaling pathways, while in the serotype D-stimulated group, the immune-related pathways were the phagosome and B cell receptor signaling pathways. Based on the predicted results of TargetScan and miRanda, the differentially expressed mRNA-miRNA network of immune-related GO terms and KEGG pathways was constructed. According to the cell morphological changes and the significant immune-related KEGG pathways, it was speculated that the serotype D strain-stimulated goat bronchial epithelial cells may induce a cellular immune response earlier than serotype A-stimulated cells. Our study provides valuable insight into the host immune response mechanism induced by strains of serotype A and D.
（）是一种动物源性细菌，主要定植于多种养殖和野生动物的呼吸道和肺部，引起严重的呼吸道疾病。为了研究血清型 A 和 D 型 菌株引起宿主免疫反应的特点，我们进行了高通量 mRNA-Seq 和 miRNA-Seq 分析，以研究这两种血清型 对山羊支气管上皮细胞刺激 4 小时后的变化。使用定量 RT-PCR 对随机选择的基因和 miRNA 进行验证。结果显示，血清型 A 和 D 刺激组分别有 204 个和 117 个差异表达的 mRNAs（|log(Fold-change)|≥1， < 0.05）。同时，差异表达的 miRNAs 数量分别为 269 个和 290 个（|log(Fold-change)|>0.1， < 0.05）。GO 和 KEGG 富集分析显示，与免疫相关的有 13 个 GO 术语（ < 0.05）和 4 个 KEGG 通路（ < 0.05）。在血清型 A 刺激组中，与免疫相关的通路是 GABA 能突触和 Toll 样受体信号通路，而在血清型 D 刺激组中，与免疫相关的通路是吞噬体和 B 细胞受体信号通路。基于 TargetScan 和 miRanda 的预测结果，构建了免疫相关 GO 术语和 KEGG 通路的差异表达 mRNA-miRNA 网络。根据细胞形态变化和显著的免疫相关 KEGG 通路，推测血清型 D 株刺激的山羊支气管上皮细胞可能比血清型 A 株更早诱导细胞免疫反应。我们的研究为血清型 A 和 D 型 菌株引起的宿主免疫反应机制提供了有价值的见解。
