Lin Z X, Wang D S, Lei S J, Wang K R
Sci Sin B. 1986 Mar;29(3):289-94.
Anti-tubulin antibody indirect immunofluorescence microscopy is used in this report. Difference of intracellular microtubule fluorescent staining patterns between normal and malignant human esophageal cells has been observed. Primarily cultured human esophageal epithelial cells display an elaborate array of fluorescent cytoplasmic microtubule filaments, the cytoplasmic microtubule complex (CMTC), in interphase cells and mitotic spindle microtubules in mitotic cells. Malignant esophageal cells from human esophageal carcinoma, ECa109 cells, contain very few cytoplasmic microtubules in interphase cells, but there is no difference of mitotic spindle microtubule fluorescence between normal and malignant cells. The results presented in this report suggest that the diminution of interphase cytoplasmic microtubules in tumor cells is probably due to the deficiency of microtubule organizing mechanism in interphase tumor cells.
本报告采用抗微管蛋白抗体间接免疫荧光显微镜技术。观察了正常和恶性人食管细胞内微管荧光染色模式的差异。原代培养的人食管上皮细胞在间期细胞中呈现出精细的荧光细胞质微管丝阵列，即细胞质微管复合体（CMTC），在有丝分裂细胞中呈现出有丝分裂纺锤体微管。人食管癌的恶性食管细胞，ECa109细胞，在间期细胞中含有极少的细胞质微管，但正常细胞和恶性细胞之间的有丝分裂纺锤体微管荧光没有差异。本报告中的结果表明，肿瘤细胞中间期细胞质微管的减少可能是由于间期肿瘤细胞中微管组织机制的缺陷。
