Zorn Savienne, Carvalho Ana, Bento Heitor, Gambarato Bruno, Pedro Guilherme, da Silva Ana, Gonçalves Rhyan, Da Rós Patrícia, Silva Messias
Engineering School of Lorena, University of São Paulo, Lorena 12602-810, SP, Brazil.
Institute of Chemistry, Federal University of Alfenas, Alfenas 37130-001, MG, Brazil.
Membranes (Basel). 2022 Feb 24;12(3):258. doi: 10.3390/membranes12030258.
In this study, a lichen-like structure was obtained through the production of a unique biomass, formed by algae cells of adhering to the mycelium of filamentous fungal This structure was composed in two steps; in the first one, microalgal cells and spores were incubated separately, and in the second one, after 72 h of growth, isolated, mature mycelium was harvested and added to cell culture. For spores' incubation, a culture medium containing only 2 g·L of glucose and minerals was used. This culture medium, with low sugar content, provided a fungal biomass to the anchorage of microalgae cells. WC medium was used without and with sugarcane molasses supplementation for microalgae cells' incubation. The lichen-type structure that was formed resulted in 99.7% efficiency in the recovery of microalgae cells and in up to 80% efficiency in the recovery of algae biomass in the lichen biomass composition. In addition, the resulting consortium attained a satisfactory lipid accumulation value (38.2 wt%) with a balanced fatty acid composition of 52.7% saturated plus monounsaturated fatty acids and 47.4% polyunsaturated fatty acids. Since fungal species are easy to recover, unlike microalgae, the lichen-like structure produced indicates an efficient low-cost bioremediation and harvesting alternative; in addition, it provides an oleaginous biomass for various industrial applications.
在本研究中，通过产生一种独特的生物质获得了一种地衣状结构，该结构由附着在丝状真菌菌丝体上的藻类细胞形成。此结构分两步构建；第一步，将微藻细胞和孢子分别培养，第二步，在生长72小时后，收获分离出的成熟菌丝体并添加到细胞培养物中。对于孢子培养，使用仅含有2 g·L葡萄糖和矿物质的培养基。这种低糖培养基为微藻细胞的附着提供了真菌生物质。微藻细胞培养使用了不添加和添加甘蔗 molasses的WC培养基。形成的地衣型结构在微藻细胞回收方面效率达到99.7%，在地衣生物质组成中藻类生物质回收效率高达80%。此外，所得共生体获得了令人满意的脂质积累值（38.2 wt%），脂肪酸组成平衡，饱和脂肪酸与单不饱和脂肪酸占52.7%，多不饱和脂肪酸占47.4%。由于真菌物种与微藻不同，易于回收，所产生的地衣状结构表明了一种高效低成本的生物修复和收获替代方法；此外，它为各种工业应用提供了含油生物质。
