真菌颗粒对微藻生物质收获的絮凝性能和机理。
Flocculation performance and mechanism of fungal pellets on harvesting of microalgal biomass.
机构信息
State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.
State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin 150090, China.
出版信息
Bioresour Technol. 2021 Feb;321:124463. doi: 10.1016/j.biortech.2020.124463. Epub 2020 Dec 1.
In this study, a bioflocculation method assisted by fungal pellets was developed for highly efficient microalgae harvesting. Effects of critical parameters, including flocculation type, temperature, rotation speed and initial pH, on the bioflocculation of fungal Aspergillus niger for microalgae Scenedesmus sp. were investigated. Results showed that the maximum flocculation efficiency of 99.4% was obtained when the fungal pellets were inoculated in the algal solution at the initial pH of 8.0, temperature of 30 °C and rotation speed of 160 rpm for 48 h in BG-11 medium. Furthermore, microscopy examination, scanning electron microscopy, Fourier transform infrared spectroscopy, Zeta potential measurement and three-dimensional excitation emission matrix fluorescence spectroscopy were used to explore the mechanism of bioflocculation process. It was found that the interaction of fungi and microalgae was related to the surface functional groups of fungal pellets. This study provides a interpretation of conceivable mechanism for microalgal bioflocculation by fungal pellets.
在这项研究中，开发了一种真菌颗粒辅助的生物絮凝方法，用于高效收获微藻。考察了关键参数，包括絮凝类型、温度、转速和初始 pH 值，对真菌黑曲霉絮凝微藻 Scenedesmus sp. 的影响。结果表明，当真菌颗粒在初始 pH 值为 8.0、温度为 30°C、转速为 160 rpm 的条件下接种到 BG-11 培养基中的藻液中，48 h 后可获得最大絮凝效率 99.4%。此外，还通过显微镜检查、扫描电子显微镜、傅里叶变换红外光谱、Zeta 电位测量和三维激发发射矩阵荧光光谱来探讨生物絮凝过程的机理。结果发现，真菌和微藻之间的相互作用与真菌颗粒的表面官能团有关。本研究为真菌颗粒絮凝微藻的可能机理提供了一种解释。
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