Luster M I, Tucker A N, Germolec D R, Silver M T, Thomas P T, Vore S J, Bucher J R
Toxicol Appl Pharmacol. 1986 Oct;86(1):140-4. doi: 10.1016/0041-008x(86)90407-2.
The effects of methyl isocyanate (MIC) on systemic immunity were evaluated in female B6C3F1 mice exposed via inhalation to 0, 1, or 3 ppm for 6 hr per day on four consecutive days. Humoral immunity, measured as the antibody response to sheep erythrocytes, and natural killer cell activity were not affected by MIC. Furthermore, resistance to the infectious agents Listeria monocytogenes, mouse malaria parasite, and influenza virus, or to B16F10 transplantable tumor cells, was not compromised by MIC exposure. Although lymphoproliferative responses to mitogens were not significantly suppressed, the response of splenic lymphocytes to allogeneic leukocytes in a mixed leukocyte response (MLR) was suppressed in a dose-related fashion and differed significantly from the control response at the 3-ppm level. These studies indicate that MIC exposure in mice does not severely alter systemic immunity. The moderate changes detected in immune function may be a secondary consequence of respiratory toxicity which occurred in these animals.
在雌性B6C3F1小鼠中评估了异氰酸甲酯（MIC）对全身免疫的影响，这些小鼠连续四天每天经吸入暴露于0、1或3 ppm的MIC中，持续6小时。以对绵羊红细胞的抗体反应衡量的体液免疫和自然杀伤细胞活性未受MIC影响。此外，对单核细胞增生李斯特菌、小鼠疟原虫、流感病毒等感染因子或对B16F10可移植肿瘤细胞的抵抗力，并未因暴露于MIC而受损。虽然对有丝分裂原的淋巴细胞增殖反应未受到显著抑制，但在混合淋巴细胞反应（MLR）中，脾淋巴细胞对同种异体白细胞的反应呈剂量相关方式受到抑制，且在3 ppm水平时与对照反应有显著差异。这些研究表明，小鼠暴露于MIC不会严重改变全身免疫。在免疫功能中检测到的适度变化可能是这些动物发生的呼吸道毒性的继发后果。
