Area of Toxicology, Faculty of Pharmacy, University of Sevilla, C/Profesor García González 2, 41012 Sevilla, Spain.
Department of Biochemistry and Molecular Biology, Faculty of Pharmacy, University of Sevilla, 41012 Sevilla, Spain.
Toxins (Basel). 2022 Feb 26;14(3):175. doi: 10.3390/toxins14030175.
Cylindrospermopsin (CYN) is a cyanotoxin whose incidence has been increasing in the last decades. Due to its capacity to exert damage at different levels of the organism, it is considered a cytotoxin. Although the main target organ is the liver, recent studies indicate that CYN has potential toxic effects on the nervous system, both in vitro and in vivo. Thus, the aim of the present work was to study the effects of this cyanotoxin on neuronal viability and synaptic integrity in murine primary cultures of neurons exposed to environmentally relevant concentrations (0-1 µg/mL CYN) for 12, 24, and 48 h. The results demonstrate a concentration- and time-dependent decrease in cell viability; no cytotoxicity was detected after exposure to the cyanotoxin for 12 h, while all of the concentrations assayed decreased this parameter after 48 h. Furthermore, CYN was also demonstrated to exert damage at the synaptic level in a murine primary neuronal culture in a concentration- and time-dependent manner. These data highlight the importance of studying the neurotoxic properties of this cyanotoxin in different experimental models.
柱孢藻毒素 (CYN) 是一种蓝藻毒素，其发病率在过去几十年中一直在增加。由于它能够在生物体的不同水平上发挥作用，因此被认为是一种细胞毒素。尽管主要靶器官是肝脏，但最近的研究表明，CYN 对神经系统具有潜在的毒性作用，无论是在体外还是体内。因此，本工作的目的是研究这种蓝藻毒素对暴露于环境相关浓度（0-1 µg/mL CYN）12、24 和 48 h 的鼠原代神经元培养物中神经元活力和突触完整性的影响。结果表明，细胞活力呈浓度和时间依赖性下降；在暴露于蓝藻毒素 12 h 后未检测到细胞毒性，而所有检测到的浓度在 48 h 后均降低了该参数。此外，还证明 CYN 以浓度和时间依赖的方式在鼠原代神经元培养物中发挥突触水平的损伤作用。这些数据强调了在不同实验模型中研究这种蓝藻毒素神经毒性特性的重要性。
