Ahmed Heba A, El Bayomi Rasha M, Hamed Rehab I, Mohsen Rasha A, El-Gohary Fatma A, Hefny Ahmed A, Elkhawaga Eman, Tolba Hala M N
Department of Zoonoses, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
Department of Food Control, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt.
Vet Sci. 2022 Mar 2;9(3):109. doi: 10.3390/vetsci9030109.
In this study, we determined the prevalence and toxin types of antibiotic-resistant in chicken, pigeons, camels, and humans. We investigated the inhibitory effects of AgNPs on biofilm formation ability of the isolates and the genetic relatedness of the isolates from various sources determined using RAPD-PCR. Fifty isolates were identified using PCR, and all the isolates were of type A. The and 2 genes were detected in 12% and 56% of the isolates, respectively. The effect of AgNPs on biofilm production of six representative isolates indicated that at the highest concentration of AgNPs (100 µg/mL), the inhibition percentages were 80.8-82.8%. The RAPD-PCR patterns of the 50 isolates from various sources revealed 33 profiles and four clusters, and the discriminatory power of RAPD-PCR was high. Multidrug-resistant isolates are predominant in the study area. The inhibition of biofilm formation by isolates was dose-dependent, and RAPD-PCR is a promising method for studying the genetic relatedness between the isolates from various sources. This is the first report of AgNPs' anti-biofilm activity against from chickens, pigeons, camels, and humans, to the best of our knowledge.
在本研究中，我们测定了鸡、鸽、骆驼和人类中耐抗生素的流行情况和毒素类型。我们研究了银纳米颗粒对分离株生物膜形成能力的抑制作用，以及使用随机扩增多态性DNA聚合酶链反应（RAPD-PCR）确定的不同来源分离株的遗传相关性。使用聚合酶链反应（PCR）鉴定出50株分离株，所有分离株均为A型。分别在12%和56%的分离株中检测到了 和2基因。银纳米颗粒对6株代表性分离株生物膜产生的影响表明，在银纳米颗粒的最高浓度（100 µg/mL）下，抑制率为80.8 - 82.8%。来自不同来源的50株 分离株的RAPD-PCR图谱显示出33种谱型和4个聚类，并且RAPD-PCR的鉴别力很高。多重耐药 分离株在研究区域占主导地位。 分离株对生物膜形成的抑制作用呈剂量依赖性，并且RAPD-PCR是研究不同来源分离株之间遗传相关性的一种有前景的方法。据我们所知，这是关于银纳米颗粒对来自鸡、鸽、骆驼和人类的 具有抗生物膜活性的首次报道。
