Osváth Péter, Szűcs Miklós, Börzsei Denise, Szabó Renáta, Lesi Zelma Nadin, Turcsán Zsolt, Veszelka Médea, Sebestyén Judith, Juhász Béla, Priksz Dániel, Varga Csaba, Pósa Anikó
Department of Physiology, Anatomy and Neuroscience, Faculty of Science and Informatics, University of Szeged, 6726 Szeged, Hungary.
Department of Urology and Andrology, Kenezy Gyula Campus, University of Debrecen, 4031 Debrecen, Hungary.
Antioxidants (Basel). 2022 Feb 22;11(3):436. doi: 10.3390/antiox11030436.
The development and progression of male infertility are closely linked to a sedentary lifestyle; however, its underlying mechanisms are not fully elucidated. Our aim was to assess the protective effects of moderate swimming exercise on the male reproductive system in isoproterenol-treated rats. Male Wistar rats were divided into five groups as follows: (1) non-interventional controls (CTRL), (2) isoproterenol-treated (ISO), (3) pre-treatment swimming training + ISO (PRE + ISO), (4) ISO + post-treatment swimming training (ISO+POST), (5) pre-treatment swimming training + ISO + post-treatment swimming training (PRE + ISO + POST) groups. Testicular oxidative stress was induced by ISO injection (1.0 mg/kg). Rats in the pre- or post-training groups were trained five days a week. At the end of the experimental period, serum testosterone levels, sperms' hyaluronan binding, and total glutathione (GSH) content, as well as myeloperoxidase activity (MPO), TNF alpha and IL6 concentrations in the testis and semen, were measured. Serum testosterone levels, sperms' hyaluronan binding, and GSH content were found to be significantly reduced, while MPO, TNF alpha and IL6 concentrations in the testis and semen were elevated after the ISO treatment compared to the CTRL group. Moderate-intensity swimming exercise effectively alleviated the negative effects of high oxidative stress. Our findings provide the first evidence that moderate-intensity swimming exercise confers sustained protection from isoproterenol-induced adverse effects on testicular inflammation.
男性不育症的发生和发展与久坐不动的生活方式密切相关；然而，其潜在机制尚未完全阐明。我们的目的是评估适度游泳运动对异丙肾上腺素处理的大鼠雄性生殖系统的保护作用。雄性Wistar大鼠分为以下五组：(1)非干预对照组(CTRL)，(2)异丙肾上腺素处理组(ISO)，(3)预处理游泳训练+ISO组(PRE+ISO)，(4)ISO+后处理游泳训练组(ISO+POST)，(5)预处理游泳训练+ISO+后处理游泳训练组(PRE+ISO+POST)。通过注射ISO(1.0mg/kg)诱导睾丸氧化应激。训练前或训练后的大鼠每周训练五天。在实验期结束时，测量血清睾酮水平、精子的透明质酸结合能力、总谷胱甘肽(GSH)含量，以及睾丸和精液中的髓过氧化物酶活性(MPO)、肿瘤坏死因子α(TNFα)和白细胞介素6(IL6)浓度。与CTRL组相比，ISO处理后血清睾酮水平、精子的透明质酸结合能力和GSH含量显著降低，而睾丸和精液中的MPO、TNFα和IL6浓度升高。中等强度的游泳运动有效减轻了高氧化应激的负面影响。我们的研究结果首次证明，中等强度的游泳运动能持续保护睾丸免受异丙肾上腺素诱导的炎症不良反应。
