Almasi Shadi, Jafarzadeh Shirazi Mohammad Reza, Rezvani Mohammad Reza, Ramezani Mahdi, Salehi Iraj, Javid-Moghadam Sasan, Komaki Alireza
Department of Animal Science, School of Agriculture, Shiraz University, Shiraz, Iran.
Department of Anatomy, School of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran.
Heliyon. 2025 Feb 4;11(4):e42468. doi: 10.1016/j.heliyon.2025.e42468. eCollection 2025 Feb 28.
Oxidative stress (OS) affects testicular function and is a significant cause of sperm cell dysfunction in males. The development of male infertility is closely linked to a sedentary lifestyle and diet. This study aimed to characterize the protective effects of biotin supplementation (BS) and swimming training (ST) on OS markers in the reproductive system of male rats. Forty male rats (200-250 g) were randomly assigned to four groups (n = 10 per group) and treated for 28 days as follows: control, BS (this group received BS through oral gavage), ST, and BS + ST groups. Our results showed that glutathione (GSH) levels significantly increased in the BS, ST (p < 0.05), and BS + ST (p < 0.01) groups, whereas malondialdehyde (MDA) levels significantly decreased in these experimental groups (p < 0.05 for all). Additionally, compared to the controls, there was a significant increase (p < 0.05) in blood levels of follicle-stimulating hormone (FSH), luteinizing hormone (LH), testosterone (T), and (HDL) in the BS and ST groups evidenced by a decrease in the levels of other biochemical parameters (cholesterol (CHO), triglyceride (TG), low-density lipoprotein (LDL), (ALT), aspartate transaminase (AST), and alkaline phosphatase (ALP)). According to histological examination, the BS, ST, and BS + ST groups showed an improvement compared to the control group. In conclusion, BS had positive effects on biochemical parameters and antioxidant activity, and BS along with ST improved testicular function in male rats.
氧化应激（OS）会影响睾丸功能，是男性精子细胞功能障碍的一个重要原因。男性不育症的发展与久坐不动的生活方式和饮食密切相关。本研究旨在表征补充生物素（BS）和游泳训练（ST）对雄性大鼠生殖系统中氧化应激标志物的保护作用。将40只雄性大鼠（200 - 250克）随机分为四组（每组n = 10），并进行如下28天的处理：对照组、BS组（该组通过灌胃接受BS）、ST组以及BS + ST组。我们的结果显示，BS组、ST组（p < 0.05）和BS + ST组（p < 0.01）中的谷胱甘肽（GSH）水平显著升高，而这些实验组中的丙二醛（MDA）水平显著降低（所有p < 0.05）。此外，与对照组相比，BS组和ST组的促卵泡生成素（FSH）、促黄体生成素（LH）、睾酮（T）以及高密度脂蛋白（HDL）的血液水平显著升高（p < 0.05），其他生化参数（胆固醇（CHO）、甘油三酯（TG）、低密度脂蛋白（LDL）、谷丙转氨酶（ALT）、天冬氨酸转氨酶（AST）和碱性磷酸酶（ALP））的水平降低。根据组织学检查，与对照组相比，BS组、ST组和BS + ST组均有改善。总之，BS对生化参数和抗氧化活性有积极影响，并且BS与ST一起改善了雄性大鼠的睾丸功能。
