• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

乙酰半胱氨酸通过非氧化形式白蛋白的再生来抑制血小板功能。

-Acetylcysteine Inhibits Platelet Function through the Regeneration of the Non-Oxidative Form of Albumin.

作者信息

Eligini Sonia, Porro Benedetta, Aldini Giancarlo, Colli Susanna, Banfi Cristina

机构信息

Centro Cardiologico Monzino I.R.C.C.S., 20138 Milan, Italy.

Dipartimento di Scienze Farmaceutiche, Università degli Studi di Milano, 20133 Milan, Italy.

出版信息

Antioxidants (Basel). 2022 Feb 23;11(3):445. doi: 10.3390/antiox11030445.

DOI:10.3390/antiox11030445
PMID:35326096
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8944739/
Abstract

-acetylcysteine (NAC) is able to break down protein disulfides, generating free thiols. This mechanism occurs on mixed disulfides of albumin (HSA) to form mercaptoalbumin (HMA), the main antioxidant species in the plasma. Circulating HSA exists in two main forms: the reduced form (HMA), and the oxidized forms, whose predominant modification is cystenylation (HSA-Cys). Increased levels of oxidized HSA have been detected in several diseases associated with oxidative stress. This study showed that NAC inhibits platelet aggregation by restoring HMA. In addition, the regeneration of HMA by NAC inhibits platelet functions such as intracellular calcium mobilization, reactive oxygen species generation, arachidonic acid metabolites synthesis, and adhesion to the collagen matrix. In our conditions, the exposure of platelets to NAC did not increase GSH levels. However, the inhibition of platelet aggregation was also detected following treatment of platelet-rich plasma with GSH, which, similarly to NAC, reduced HSA-Cys levels. Furthermore, this study showed that cysteine, another compound able to restore HMA by reducing the HSA-Cys content, inhibited platelet aggregation to a similar extent as NAC. The results obtained in this study suggest a new mechanism by which NAC can modulate platelet activation and suggest its possible use as an antiplatelet drug in conditions associated with oxidative stress.

摘要

乙酰半胱氨酸（NAC）能够分解蛋白质二硫键，生成游离巯基。这一机制发生在白蛋白（HSA）的混合二硫键上，形成血浆中的主要抗氧化物质巯基白蛋白（HMA）。循环中的HSA主要以两种形式存在：还原形式（HMA）和氧化形式，其主要修饰为半胱氨酸化（HSA-Cys）。在几种与氧化应激相关的疾病中，已检测到氧化型HSA水平升高。本研究表明，NAC通过恢复HMA来抑制血小板聚集。此外，NAC使HMA再生可抑制血小板功能，如细胞内钙动员、活性氧生成、花生四烯酸代谢物合成以及与胶原基质的黏附。在我们的实验条件下，血小板暴露于NAC并未增加谷胱甘肽（GSH）水平。然而，用GSH处理富血小板血浆后也检测到了血小板聚集的抑制，与NAC类似，GSH也降低了HSA-Cys水平。此外，本研究表明，另一种能够通过降低HSA-Cys含量来恢复HMA的化合物半胱氨酸，其抑制血小板聚集的程度与NAC相似。本研究获得的结果提示了一种NAC调节血小板活化的新机制，并表明其在与氧化应激相关的疾病中可能作为一种抗血小板药物使用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/efe9acab5016/antioxidants-11-00445-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/139604327283/antioxidants-11-00445-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/a7991b13e20f/antioxidants-11-00445-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/8c8c20b36f64/antioxidants-11-00445-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/54cd91f609b3/antioxidants-11-00445-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/e37385a30e4f/antioxidants-11-00445-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/f2eaa35415df/antioxidants-11-00445-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/f3e02a5a8ea5/antioxidants-11-00445-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/efe9acab5016/antioxidants-11-00445-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/139604327283/antioxidants-11-00445-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/a7991b13e20f/antioxidants-11-00445-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/8c8c20b36f64/antioxidants-11-00445-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/54cd91f609b3/antioxidants-11-00445-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/e37385a30e4f/antioxidants-11-00445-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/f2eaa35415df/antioxidants-11-00445-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/f3e02a5a8ea5/antioxidants-11-00445-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f2e/8944739/efe9acab5016/antioxidants-11-00445-g008.jpg

相似文献

1
-Acetylcysteine Inhibits Platelet Function through the Regeneration of the Non-Oxidative Form of Albumin.乙酰半胱氨酸通过非氧化形式白蛋白的再生来抑制血小板功能。
Antioxidants (Basel). 2022 Feb 23;11(3):445. doi: 10.3390/antiox11030445.
2
N-Acetylcysteine Regenerates In Vivo Mercaptoalbumin.N-乙酰半胱氨酸可在体内使巯基白蛋白再生。
Antioxidants (Basel). 2022 Sep 6;11(9):1758. doi: 10.3390/antiox11091758.
3
Kinetic studies of covalent binding between N-acetyl-L-cysteine and human serum albumin through a mixed-disulfide using an N-methylpyridinium polymer-based column.使用基于N-甲基吡啶鎓聚合物的柱子，通过混合二硫键对N-乙酰-L-半胱氨酸与人血清白蛋白之间的共价结合进行动力学研究。
Drug Metab Pharmacokinet. 2004 Aug;19(4):297-302. doi: 10.2133/dmpk.19.297.
4
Therapeutic potential of N-acetylcysteine as an antiplatelet agent in patients with type-2 diabetes.N-乙酰半胱氨酸作为抗血小板药物在 2 型糖尿病患者中的治疗潜力。
Cardiovasc Diabetol. 2011 May 21;10:43. doi: 10.1186/1475-2840-10-43.
5
Evaluation of the antioxidant properties of N-acetylcysteine in human platelets: prerequisite for bioconversion to glutathione for antioxidant and antiplatelet activity.评价 N-乙酰半胱氨酸在人血小板中的抗氧化特性：生物转化为谷胱甘肽以发挥抗氧化和抗血小板活性的前提。
J Cardiovasc Pharmacol. 2009 Oct;54(4):319-26. doi: 10.1097/FJC.0b013e3181b6e77b.
6
HPLC separation of human serum albumin isoforms based on their isoelectric points.基于等电点的人血清白蛋白异构体的 HPLC 分离。
J Chromatogr B Analyt Technol Biomed Life Sci. 2014 Jan 1;944:144-151. doi: 10.1016/j.jchromb.2013.11.019. Epub 2013 Nov 20.
7
N-Acetylcysteine, N-Acetylcysteine Amide, and Thioredoxin Mimetic Peptides Regenerate Mercaptoalbumin and Exhibit Antioxidant Activity.N-乙酰半胱氨酸、N-乙酰半胱氨酸酰胺和硫氧还蛋白模拟肽可使巯基白蛋白再生并具有抗氧化活性。
Antioxidants (Basel). 2024 Mar 15;13(3):351. doi: 10.3390/antiox13030351.
8
N-Acetylcysteine as an antioxidant and disulphide breaking agent: the reasons why.N-乙酰半胱氨酸作为一种抗氧化剂和二硫键断裂剂：原因如下。
Free Radic Res. 2018 Jul;52(7):751-762. doi: 10.1080/10715762.2018.1468564. Epub 2018 May 9.
9
N -Acetyl-cysteine reduces homocysteine plasma levels after single intravenous administration by increasing thiols urinary excretion.单次静脉注射后，N-乙酰半胱氨酸通过增加尿中硫醇排泄量来降低血浆同型半胱氨酸水平。
Pharmacol Res. 1999 Oct;40(4):345-50. doi: 10.1006/phrs.1999.0519.
10
N-acetylcysteine Amide AD4/NACA and Thioredoxin Mimetic Peptides Inhibit Platelet Aggregation and Protect against Oxidative Stress.N-乙酰半胱氨酸酰胺AD4/ NACA和硫氧还蛋白模拟肽可抑制血小板聚集并抵御氧化应激。
Antioxidants (Basel). 2023 Jul 7;12(7):1395. doi: 10.3390/antiox12071395.

引用本文的文献

1
Effect of N-Acetylcysteine on mortality in COVID-19 patients: A systematic review and meta-analysis of randomized controlled trials.N-乙酰半胱氨酸对COVID-19患者死亡率的影响：一项随机对照试验的系统评价和荟萃分析
Inflammopharmacology. 2025 Aug;33(8):4871-4877. doi: 10.1007/s10787-025-01876-x. Epub 2025 Jul 29.
2
The Reactive Oxygen Species Scavenger N-Acetyl-L-Cysteine Reduces Storage-Dependent Decline in Integrin -Mediated Platelet Function, Inhibiting Pre-Activation of Integrin and Its Subunit Cleavage.活性氧清除剂N-乙酰-L-半胱氨酸可减少整合素介导的血小板功能的储存依赖性下降，抑制整合素的预激活及其亚基裂解。
Oxid Med Cell Longev. 2025 Apr 21;2025:7499648. doi: 10.1155/omcl/7499648. eCollection 2025.
3

本文引用的文献

1
-Acetylcysteine (NAC): Impacts on Human Health.-乙酰半胱氨酸（NAC）：对人类健康的影响。
Antioxidants (Basel). 2021 Jun 16;10(6):967. doi: 10.3390/antiox10060967.
2
Platelet adhesion in type 2 diabetes: impact of plasma albumin and mean platelet volume.2型糖尿病中的血小板黏附：血浆白蛋白和平均血小板体积的影响
Thromb J. 2021 Jun 2;19(1):40. doi: 10.1186/s12959-021-00291-w.
3
The multifaceted contribution of platelets in the emergence and aftermath of acute cardiovascular events.血小板在急性心血管事件发生前后的多方面贡献。
Platelet Reactive Oxygen Species, Oxidised Lipid Stress, Current Perspectives, and an Update on Future Directions.
血小板活性氧、氧化脂质应激、当前观点及未来方向的最新进展
Cells. 2025 Mar 27;14(7):500. doi: 10.3390/cells14070500.
4
Reactive oxygen species: Orchestrating the delicate dance of platelet life and death.活性氧：编排血小板生死的微妙舞蹈。
Redox Biol. 2025 Mar;80:103489. doi: 10.1016/j.redox.2025.103489. Epub 2025 Jan 3.
5
The potential treatment of N-acetylcysteine as an antioxidant in the radiation-induced heart disease.N-乙酰半胱氨酸作为抗氧化剂在放射性心脏病中的潜在治疗作用。
Cardiovasc Diagn Ther. 2024 Aug 31;14(4):509-524. doi: 10.21037/cdt-24-19. Epub 2024 Aug 21.
6
N-Acetylcysteine, N-Acetylcysteine Amide, and Thioredoxin Mimetic Peptides Regenerate Mercaptoalbumin and Exhibit Antioxidant Activity.N-乙酰半胱氨酸、N-乙酰半胱氨酸酰胺和硫氧还蛋白模拟肽可使巯基白蛋白再生并具有抗氧化活性。
Antioxidants (Basel). 2024 Mar 15;13(3):351. doi: 10.3390/antiox13030351.
7
Albumin Thiolation and Oxidative Stress Status in Patients with Aortic Valve Stenosis.白蛋白巯基化与主动脉瓣狭窄患者氧化应激状态。
Biomolecules. 2023 Nov 28;13(12):1713. doi: 10.3390/biom13121713.
8
N-acetylcysteine Amide AD4/NACA and Thioredoxin Mimetic Peptides Inhibit Platelet Aggregation and Protect against Oxidative Stress.N-乙酰半胱氨酸酰胺AD4/ NACA和硫氧还蛋白模拟肽可抑制血小板聚集并抵御氧化应激。
Antioxidants (Basel). 2023 Jul 7;12(7):1395. doi: 10.3390/antiox12071395.
9
Sex Differences in X-ray-Induced Endothelial Damage: Effect of Taurine and N-Acetylcysteine.X射线诱导的内皮损伤中的性别差异：牛磺酸和N-乙酰半胱氨酸的作用。
Antioxidants (Basel). 2022 Dec 29;12(1):77. doi: 10.3390/antiox12010077.
10
N-Acetylcysteine Regenerates In Vivo Mercaptoalbumin.N-乙酰半胱氨酸可在体内使巯基白蛋白再生。
Antioxidants (Basel). 2022 Sep 6;11(9):1758. doi: 10.3390/antiox11091758.
Atherosclerosis. 2021 Feb;319:132-141. doi: 10.1016/j.atherosclerosis.2020.12.017. Epub 2021 Jan 16.
4
The Multifaceted Therapeutic Role of N-Acetylcysteine (NAC) in Disorders Characterized by Oxidative Stress.N-乙酰半胱氨酸（NAC）在氧化应激相关疾病中的多效治疗作用。
Curr Neuropharmacol. 2021;19(8):1202-1224. doi: 10.2174/1570159X19666201230144109.
5
N-Acetyl Cysteine Targets Hepatic Lipid Accumulation to Curb Oxidative Stress and Inflammation in NAFLD: A Comprehensive Analysis of the Literature.N-乙酰半胱氨酸靶向肝脏脂质蓄积以抑制非酒精性脂肪性肝病中的氧化应激和炎症：文献综述
Antioxidants (Basel). 2020 Dec 16;9(12):1283. doi: 10.3390/antiox9121283.
6
S-Thiolation Targets Albumin in Heart Failure.S-硫醇化作用以心力衰竭中的白蛋白为靶点。
Antioxidants (Basel). 2020 Aug 17;9(8):763. doi: 10.3390/antiox9080763.
7
Serum albumin and cardiovascular disease: State-of-the-art review.血清白蛋白与心血管疾病：最新综述。
Ann Cardiol Angeiol (Paris). 2020 Oct;69(4):192-200. doi: 10.1016/j.ancard.2020.07.012. Epub 2020 Aug 11.
8
-Acetyl-Cysteine Regenerates Albumin Cys34 by a Thiol-Disulfide Breaking Mechanism: An Explanation of Its Extracellular Antioxidant Activity.-乙酰半胱氨酸通过硫醇-二硫键断裂机制使白蛋白Cys34再生：对其细胞外抗氧化活性的一种解释。
Antioxidants (Basel). 2020 Apr 28;9(5):367. doi: 10.3390/antiox9050367.
9
Hyperoxidized Albumin Modulates Platelets and Promotes Inflammation Through CD36 Receptor in Severe Alcoholic Hepatitis.高氧化白蛋白通过CD36受体调节血小板并促进重症酒精性肝炎中的炎症反应。
Hepatol Commun. 2019 Nov 23;4(1):50-65. doi: 10.1002/hep4.1440. eCollection 2020 Jan.
10
Oxidized Albumin Triggers a Cytokine Storm in Leukocytes Through P38 Mitogen-Activated Protein Kinase: Role in Systemic Inflammation in Decompensated Cirrhosis.氧化白蛋白通过 p38 丝裂原活化蛋白激酶触发白细胞细胞因子风暴：失代偿期肝硬化全身炎症中的作用。
Hepatology. 2018 Nov;68(5):1937-1952. doi: 10.1002/hep.30135. Epub 2018 Oct 13.