Hu Shuyang, Li Yuwei, Wang Boran, Yin Lijun, Jia Xin
College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China.
Foods. 2022 Mar 15;11(6):840. doi: 10.3390/foods11060840.
The growth of bacteria is affected by environmental conditions, and unfavorable conditions will produce different degrees of stress on . The cells respond to the stress on the bacteria through changes in biological characteristics and transcriptomes. To study the effect of NaCl concentration on , we have determined the biological characteristics of the 0%, 1%, 2%, 3%, 5%, and 7% NaCl concentrations cultured to research the salt stress to bacteria. We found that the biological properties of cultured with different NaCl concentrations were different, and the expression of outer membrane proteins of changed when it was grown under different NaCl concentrations. When bacteria cultured in higher NaCl concentrations (3%, 5% and 7% NaCl), the sodium-type flagellar protein MotY was found. Finally, the transcriptome analysis of cultured with 0% NaCl and 7% NaCl was carried out to find out the differentially expressed genes. We found that the same gene have opposite up-regulated and down-regulated expression in two treatments, indicating that these types of genes are regulated different in low and high osmotic stress.
细菌的生长受环境条件影响，不利条件会对其产生不同程度的压力。细胞通过生物学特性和转录组的变化来应对细菌所受的压力。为研究NaCl浓度对[细菌名称未给出]的影响，我们测定了在0%、1%、2%、3%、5%和7% NaCl浓度下培养的[细菌名称未给出]的生物学特性，以研究盐胁迫对细菌的影响。我们发现，在不同NaCl浓度下培养的[细菌名称未给出]的生物学特性不同，且当它在不同NaCl浓度下生长时，其外膜蛋白的表达会发生变化。当细菌在较高NaCl浓度（3%、5%和7% NaCl）下培养时，发现了钠型鞭毛蛋白MotY。最后，对在0% NaCl和7% NaCl条件下培养的[细菌名称未给出]进行了转录组分析，以找出差异表达基因。我们发现，同一基因在两种处理中呈现相反的上调和下调表达，表明这些类型的基因在低渗和高渗胁迫下的调控方式不同。
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