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质体和核质中钙调节的分子网络对脱落酸和茉莉酸甲酯长期处理的响应。

Cytosolic and Nucleosolic Calcium-Regulated Molecular Networks in Response to Long-Term Treatment with Abscisic Acid and Methyl Jasmonate in .

机构信息

Beijing Key Laboratory of Gene Resource and Molecular Development, College of Life Sciences, Beijing Normal University, Beijing 100875, China.

Department of Computational Biology, Beijing Computing Center, Beijing 100094, China.

出版信息

Genes (Basel). 2022 Mar 16;13(3):524. doi: 10.3390/genes13030524.

DOI:10.3390/genes13030524
PMID:35328077
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8950999/
Abstract

Calcium acts as a universal secondary messenger that transfers developmental cues and stress signals for gene expression and adaptive growth. A prior study showed that abiotic stresses induce mutually independent cytosolic Ca ([Ca]) and nucleosolic Ca ([Ca]) increases in root cells. However, gene expression networks deciphering [Ca] and [Ca] signalling pathways remain elusive. Here, using transgenic to selectively impair abscisic acid (ABA)- or methyl jasmonate (MeJA)-induced [Ca] and [Ca] increases, we identified [Ca]- and [Ca]-regulated ABA- or MeJA-responsive genes with a genome oligo-array. Gene co-expression network analysis revealed four Ca signal-decoding genes, , , , , as hub genes co-expressed with Ca-regulated hormone-responsive genes and hormone signalling genes. Luciferase complementation imaging assays showed interactions among CAM1, CIPK8, and GAD1; they also showed interactions with several proteins encoded by Ca-regulated hormone-responsive genes. Furthermore, CAM1 and CIPK8 were required for MeJA-induced stomatal closure; they were associated with ABA-inhibited seed germination. Quantitative reverse transcription polymerase chain reaction analysis showed the unique expression pattern of [Ca]-regulated hormone-responsive genes in , , and . This comprehensive understanding of distinct Ca and hormonal signalling will allow the application of approaches to uncover novel molecular foundations for responses to developmental and stress signals in plants.

摘要

钙作为一种普遍的第二信使，将发育线索和应激信号传递到基因表达和适应性生长中。先前的研究表明，非生物胁迫会诱导根细胞中细胞质 Ca([Ca])和核仁 Ca([Ca])的独立增加。然而，破译 Ca 和 Ca 信号通路的基因表达网络仍然难以捉摸。在这里，我们使用转基因 选择性地削弱脱落酸（ABA）或茉莉酸甲酯（MeJA）诱导的 [Ca]和 [Ca]增加，使用基因组寡核苷酸阵列鉴定了 Ca 调节的 ABA 或 MeJA 响应基因。基因共表达网络分析显示了四个 Ca 信号解码基因、、、、，作为与 Ca 调节的激素响应基因和激素信号基因共表达的枢纽基因。荧光素酶互补成像测定表明 CAM1、CIPK8 和 GAD1 之间存在相互作用；它们还与 Ca 调节的激素响应基因编码的几种蛋白质存在相互作用。此外，CAM1 和 CIPK8 是 MeJA 诱导的气孔关闭所必需的；它们与 ABA 抑制的种子萌发有关。定量反转录聚合酶链反应分析显示，[Ca]调节的激素响应基因在 、、和 中具有独特的表达模式。对不同 Ca 和激素信号的综合理解将允许应用方法揭示植物对发育和应激信号响应的新分子基础。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df4a/8950999/40e338171bbd/genes-13-00524-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df4a/8950999/bdc6401dc105/genes-13-00524-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df4a/8950999/c9da43661527/genes-13-00524-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df4a/8950999/246c46643091/genes-13-00524-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df4a/8950999/38adc04e30c8/genes-13-00524-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df4a/8950999/cc766817162a/genes-13-00524-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df4a/8950999/40e338171bbd/genes-13-00524-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df4a/8950999/bdc6401dc105/genes-13-00524-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df4a/8950999/c9da43661527/genes-13-00524-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df4a/8950999/246c46643091/genes-13-00524-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df4a/8950999/38adc04e30c8/genes-13-00524-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df4a/8950999/cc766817162a/genes-13-00524-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/df4a/8950999/40e338171bbd/genes-13-00524-g006.jpg

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