Chapelle Nicolas, Osmola Malgorzata, Martin Jérôme, Blin Justine, Leroy Maxime, Jirka Iva, Moussata Driffa, Lamarque Dominique, Olivier Raphael, Tougeron David, Hay-Lombardie Anne, Bigot-Corbel Edith, Masson Damien, Mosnier Jean-François, Matysiak-Budnik Tamara
IMAD, Hepato-Gastroenterology & Digestive Oncology, University Hospital of Nantes, Hôtel Dieu, Place Alexis Ricordeau, CEDEX 1, 44093 Nantes, France.
INSERM U1064 CRTI, 44093 Nantes, France.
Diagnostics (Basel). 2022 Mar 12;12(3):695. doi: 10.3390/diagnostics12030695.
Analysis of serum biomarkers for the assessment of atrophic gastritis (AG), a gastric precancerous lesion, is of growing interest for identification of patients at increased risk of gastric cancer. The aim was to analyze the diagnostic performance of serum pepsinogen testing using another method, chemiluminescent enzyme immunoassay (CLEIA), as well as of other new potential biomarkers.
The sera of patients considered at increased risk of gastric cancer and undergoing upper endoscopy collected in our previous prospective, multicenter study were tested for pepsinogen I (PGI) and II (PGII), interleukin-6 (IL-6), human epididymal protein 4 (HE-4), adiponectin, ferritin and Krebs von den Lungen (KL-6) using the CLEIA. The diagnostic performance for the detection of AG was calculated by taking histology as the reference.
In total, 356 patients (162 men (46%); mean age 58.6 (±14.2) years), including 152 with AG, were included. For the detection of moderate to severe corpus AG, sensitivity and specificity of the pepsinogen I/II ratio were of 75.0% (95%CI 57.8-87.9) and 92.6% (88.2-95.8), respectively. For the detection of moderate to severe antrum AG, sensitivity of IL-6 was of 72.2% (95%CI 46.5-90.3). Combination of pepsinogen I/II ratio or HE-4 showed a sensitivity of 85.2% (95%CI 72.9-93.4) for the detection of moderate to severe AG at any location.
This study shows that PG testing by CLEIA represents an accurate assay for the detection of corpus AG. Additionally, IL-6 and HE-4 may be of interest for the detection of antrum AG.
MINI-ABSTRACT: Pepsinogens testing by chemiluminescent enzyme immunoassay is accurate for the detection of corpus atrophic gastritis. IL-6 and HE-4 maybe of interest for the detection of antrum atrophic gastritis.
分析血清生物标志物以评估萎缩性胃炎（AG）这一胃癌前病变，对于识别胃癌风险增加的患者越来越受到关注。目的是使用化学发光酶免疫分析法（CLEIA）等另一种方法分析血清胃蛋白酶原检测以及其他新的潜在生物标志物的诊断性能。
在我们之前的前瞻性多中心研究中收集的被认为有胃癌风险且接受上消化道内镜检查的患者血清，使用CLEIA检测胃蛋白酶原I（PGI）和II（PGII）、白细胞介素-6（IL-6）、人附睾蛋白4（HE-4）、脂联素、铁蛋白和克雷伯斯-冯-登-卢根蛋白（KL-6）。以组织学为参考计算检测AG的诊断性能。
总共纳入了356例患者（162例男性（46%）；平均年龄58.6（±14.2）岁），其中152例患有AG。对于检测中度至重度胃体部AG，胃蛋白酶原I/II比值的敏感性和特异性分别为75.0%（95%CI 57.8 - 87.9）和92.6%（88.2 - 95.8）。对于检测中度至重度胃窦部AG，IL-6的敏感性为72.2%（95%CI 46.5 - 90.3）。胃蛋白酶原I/II比值或HE-4的组合在检测任何部位的中度至重度AG时显示出85.2%（95%CI 72.9 - 93.4）的敏感性。
本研究表明，通过CLEIA进行的PG检测是检测胃体部AG的准确方法。此外，IL-6和HE-4可能对检测胃窦部AG有意义。
通过化学发光酶免疫分析法检测胃蛋白酶原对于检测胃体部萎缩性胃炎是准确的。IL-6和HE-4可能对检测胃窦部萎缩性胃炎有意义。
