Institute of Applied Biology, Shanxi University, Taiyuan 030006, China.
College of Life Science, Shanxi University, Taiyuan 030006, China.
Int J Mol Sci. 2022 Mar 13;23(6):3106. doi: 10.3390/ijms23063106.
Insect wing consists of a double layer of epidermal cells that produce and secrete the dorsal and ventral cuticular components. It is important for the stability of epidermal cells during wing development and morphogenesis, but its specific gene expression and physiological function during this process remain unclear. In our previous work, a wing cuticle protein gene was identified in based on transcriptomic data. Here, we report on its roles in wing development and morphogenesis. encodes a chitin-binding protein belonging to RR-2 subfamily of CPR family, which is highly homologous to CP19-like proteins in other insect species. RT-qPCR analysis revealed that is highly expressed in wing pads of fifth-instar nymphs, and its encoded protein is located in two layers of epidermal cells but not in the cuticle. Suppression of by RNA interference led to abnormal wing pad and wing morphogenesis with curved, unclosed, and wrinkled phenotypes during nymph-to-nymph and nymph-to-adult transition, respectively. Furthermore, deficiency of affected arrangement of epidermal cells, resulting in apoptosis. Our results indicate that is indispensable for wing development and normal morphological structure by maintaining the stability of epidermal cells during molting.
昆虫翅膀由产生和分泌背侧和腹侧表皮成分的双层表皮细胞组成。在翅膀发育和形态发生过程中，表皮细胞的稳定性很重要，但在这个过程中其特定的基因表达和生理功能仍不清楚。在我们之前的工作中，基于转录组数据，在 中鉴定出一个翅膀表皮蛋白基因 。在这里，我们报告了它在翅膀发育和形态发生中的作用。 编码一种几丁质结合蛋白，属于 CPR 家族的 RR-2 亚家族，与其他昆虫物种中的 CP19 样蛋白高度同源。RT-qPCR 分析显示， 在第五龄若虫的翅垫中高度表达，其编码的蛋白质位于两层表皮细胞中，但不在表皮中。通过 RNA 干扰抑制 导致若虫到若虫和若虫到成虫过渡期间翅膀垫和翅膀形态发生异常，出现弯曲、未闭合和起皱的表型。此外， 的缺乏会影响表皮细胞的排列，导致细胞凋亡。我们的结果表明， 在蜕皮过程中通过维持表皮细胞的稳定性，对翅膀发育和正常形态结构是必不可少的。
