Regulatory Mechanism of Transcription Factor Modulates Transcriptional Expression Enhancing Heat Tolerance in (Coleoptera: Chrysomelidae).

is a classical biological agent used to control alligator weed (). Previous research has indicated that the heat shock factor (HSF) is involved in regulating the transcriptional expression of in response to heat resistance in However, the regulatory mechanism by which regulates the expression of remains largely unknown. Here, we identified and cloned a 944 bp promoter (p) region from Subsequent bioinformatics analysis revealed that the p sequence contains multiple functional elements and has a common TATA box approximately 30 bp upstream of the transcription start site, with transcription commencing at a purine base approximately 137 bp upstream of ATG. Promoter deletion analyses revealed that the sequence from -944 to -744 bp was the core regulatory region. A dual-luciferase reporter assay indicated that overexpressed significantly enhanced the activity of p. Furthermore, qPCR showed that expression increased with time in (Sf9) cells, and overexpression significantly upregulated expression in vitro. Characterization of the upstream regulatory mechanisms demonstrated that binds to upstream -acting elements in the promoter region of from -944 to -744 bp to activate the AhHSF-AhHSP pathway at the transcriptional level to protect from high temperature damage. Furthermore, we proposed a molecular model of modulation of transcription following heat shock in . The findings of this study suggest that enhancing the heat tolerance of by modulating the upstream pathways of the family can improve the biocontrol of .